8C81
Cryo-EM structure of the yeast SPT-Orm1-Sac1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein ORM1 | polymer | 222 | 25221.7 | 1 | UniProt (P53224) Pfam (PF04061) | Saccharomyces cerevisiae (baker's yeast) | |
| 2 | B (B) | Serine palmitoyltransferase 1 | polymer | 580 | 64985.5 | 1 | UniProt (P25045) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 1,SPT1,Long chain base biosynthesis protein 1 |
| 3 | C (C) | Serine palmitoyltransferase 2 | polymer | 561 | 63189.7 | 1 | UniProt (P40970) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 2,Long chain base biosynthesis protein 2 |
| 4 | D (D) | Serine palmitoyltransferase-regulating protein TSC3 | polymer | 80 | 9590.2 | 1 | UniProt (Q3E790) | Saccharomyces cerevisiae (baker's yeast) | Temperature-sensitive CSG2-mutant suppressor protein 3 |
| 5 | E (E) | Phosphatidylinositol-3-phosphatase SAC1 | polymer | 623 | 71214.4 | 1 | UniProt (P32368) Pfam (PF02383) | Saccharomyces cerevisiae (baker's yeast) | Phosphatidylinositol-4-phosphate phosphatase,Recessive suppressor of secretory defect |
| 6 | F, I, J (A, B) | ERGOSTEROL | non-polymer | 396.6 | 3 | Chemie (ERG) PubChem (16394547) PubChem (16403935) PubChem (16405182) PubChem (21139765) PubChem (21775588) PubChem (22298561) PubChem (177562045) PubChem (1742286) PubChem (40469696) PubChem (40469697) PubChem (40469698) PubChem (40469699) PubChem (40826225) PubChem (40826226) PubChem (45044427) PubChem (69473585) PubChem (101703) PubChem (643780) PubChem (46936866) PubChem (5771570) PubChem (6475768) PubChem (122700025) PubChem (124099377) PubChem (53298316) PubChem (124421304) PubChem (10250169) PubChem (57182145) PubChem (12304302) PubChem (142431933) PubChem (57508500) PubChem (163000929) PubChem (163000920) PubChem (163000938) PubChem (164664366) PubChem (58992544) PubChem (171157824) PubChem (59373909) PubChem (59738932) PubChem (60113883) PubChem (66824684) PubChem (69866435) PubChem (70544775) PubChem (71073084) PubChem (86575818) PubChem (90690511) PubChem (90690567) PubChem (91171783) PubChem (91345764) PubChem (91375317) PubChem (91459231) PubChem (102514697) PubChem (117749797) PubChem (121358225) PubChem (5996) PubChem (123930382) PubChem (247705) PubChem (124303809) PubChem (124351226) PubChem (124427258) PubChem (124488022) PubChem (439550) PubChem (444679) PubChem (481705) PubChem (638020) PubChem (124897130) PubChem (124897142) PubChem (124897144) PubChem (124897147) PubChem (5317174) PubChem (5319551) PubChem (5351178) PubChem (5351845) PubChem (71122427) PubChem (124918943) PubChem (124918944) PubChem (124928616) PubChem (124928617) PubChem (125033743) PubChem (125033744) PubChem (125126218) PubChem (125126219) PubChem (6428692) PubChem (6432563) PubChem (6433143) PubChem (6436872) PubChem (6452576) PubChem (6713013) PubChem (6713713) PubChem (126626805) PubChem (126727349) PubChem (126727350) PubChem (7067891) PubChem (7067892) PubChem (7067893) PubChem (7067894) PubChem (7094294) PubChem (9843722) PubChem (9908757) PubChem (9930654) PubChem (9940587) PubChem (10982177) PubChem (11014783) PubChem (11269488) PubChem (129316836) PubChem (129630206) PubChem (12304304) PubChem (12304306) PubChem (12304309) PubChem (12304314) PubChem (12304315) PubChem (131632336) PubChem (134279700) PubChem (142281730) PubChem (145082804) PubChem (146230523) PubChem (154496532) PubChem (161146965) PubChem (162645168) PubChem (163000919) PubChem (163000921) PubChem (163000922) PubChem (163000923) PubChem (163000924) PubChem (163000925) PubChem (163000926) PubChem (163000927) PubChem (163000928) PubChem (163000930) PubChem (163000931) PubChem (163000932) PubChem (163000933) PubChem (163000934) PubChem (163000935) PubChem (163000936) PubChem (163000937) PubChem (163000939) PubChem (163524814) PubChem (171154016) PubChem (175800618) | |||
| 7 | G (A) | N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide | non-polymer | 696.2 | 1 | Chemie (Z8A) PubChem (74335554) PubChem (89187224) PubChem (10417280) PubChem (162876144) PubChem (170583368) | |||
| 8 | H (A) | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside | non-polymer | 1165.3 | 1 | Chemie (Q7G) | |||
| 9 | K (C) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) PubChem (1051) |
Sequence modifications
A: 1 - 222 (UniProt: P53224)
B: -21 - 558 (UniProt: P25045)
| PDB | External Database | Details |
|---|---|---|
| Ala 51 | Ser 51 | engineered mutation |
| Ala 52 | Ser 52 | engineered mutation |
| Ala 53 | Ser 53 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp -12 | - | insertion |
| Tyr -11 | - | insertion |
| Lys -10 | - | insertion |
| Asp -9 | - | insertion |
| His -8 | - | insertion |
| Asp -7 | - | insertion |
| Gly -6 | - | insertion |
| Asp -5 | - | insertion |
| Tyr -4 | - | insertion |
| Lys -3 | - | insertion |
| Asp -2 | - | insertion |
| His -1 | - | insertion |
| Asp 0 | - | insertion |
| Ile 1 | - | insertion |
| Asp 2 | - | insertion |
| Tyr 3 | - | insertion |
| Lys 4 | - | insertion |
| Asp 5 | - | insertion |
| Asp 6 | - | insertion |
| Asp 7 | - | insertion |
| Asp 8 | - | insertion |
| Lys 9 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 234201.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 3298.6 | |
| All* | Total formula weight | 237500.1 |
