8C81
Cryo-EM structure of the yeast SPT-Orm1-Sac1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein ORM1 | polymer | 222 | 25221.7 | 1 | UniProt (P53224) Pfam (PF04061) | Saccharomyces cerevisiae (baker's yeast) | |
| 2 | B (B) | Serine palmitoyltransferase 1 | polymer | 580 | 64985.5 | 1 | UniProt (P25045) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 1,SPT1,Long chain base biosynthesis protein 1 |
| 3 | C (C) | Serine palmitoyltransferase 2 | polymer | 561 | 63189.7 | 1 | UniProt (P40970) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 2,Long chain base biosynthesis protein 2 |
| 4 | D (D) | Serine palmitoyltransferase-regulating protein TSC3 | polymer | 80 | 9590.2 | 1 | UniProt (Q3E790) | Saccharomyces cerevisiae (baker's yeast) | Temperature-sensitive CSG2-mutant suppressor protein 3 |
| 5 | E (E) | Phosphatidylinositol-3-phosphatase SAC1 | polymer | 623 | 71214.4 | 1 | UniProt (P32368) Pfam (PF02383) | Saccharomyces cerevisiae (baker's yeast) | Phosphatidylinositol-4-phosphate phosphatase,Recessive suppressor of secretory defect |
| 6 | F, I, J (A, B) | ERGOSTEROL | non-polymer | 396.6 | 3 | Chemie (ERG) | |||
| 7 | G (A) | N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide | non-polymer | 696.2 | 1 | Chemie (Z8A) | |||
| 8 | H (A) | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside | non-polymer | 1165.3 | 1 | Chemie (Q7G) | |||
| 9 | K (C) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) |
Sequence modifications
A: 1 - 222 (UniProt: P53224)
B: -21 - 558 (UniProt: P25045)
| PDB | External Database | Details |
|---|---|---|
| Ala 51 | Ser 51 | engineered mutation |
| Ala 52 | Ser 52 | engineered mutation |
| Ala 53 | Ser 53 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp -12 | - | insertion |
| Tyr -11 | - | insertion |
| Lys -10 | - | insertion |
| Asp -9 | - | insertion |
| His -8 | - | insertion |
| Asp -7 | - | insertion |
| Gly -6 | - | insertion |
| Asp -5 | - | insertion |
| Tyr -4 | - | insertion |
| Lys -3 | - | insertion |
| Asp -2 | - | insertion |
| His -1 | - | insertion |
| Asp 0 | - | insertion |
| Ile 1 | - | insertion |
| Asp 2 | - | insertion |
| Tyr 3 | - | insertion |
| Lys 4 | - | insertion |
| Asp 5 | - | insertion |
| Asp 6 | - | insertion |
| Asp 7 | - | insertion |
| Asp 8 | - | insertion |
| Lys 9 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 234201.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 3298.6 | |
| All* | Total formula weight | 237500.1 |
