8C81

Cryo-EM structure of the yeast SPT-Orm1-Sac1 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0006672	biological_process	ceramide metabolic process
A	0006986	biological_process	response to unfolded protein
A	0016020	cellular_component	membrane
A	0017059	cellular_component	serine palmitoyltransferase complex
A	0030148	biological_process	sphingolipid biosynthetic process
A	0090155	biological_process	negative regulation of sphingolipid biosynthetic process
A	0090156	biological_process	intracellular sphingolipid homeostasis
B	0004758	molecular_function	serine C-palmitoyltransferase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0006665	biological_process	sphingolipid metabolic process
B	0009058	biological_process	biosynthetic process
B	0016020	cellular_component	membrane
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0017059	cellular_component	serine palmitoyltransferase complex
B	0030148	biological_process	sphingolipid biosynthetic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0046512	biological_process	sphingosine biosynthetic process
B	0046513	biological_process	ceramide biosynthetic process
B	0090156	biological_process	intracellular sphingolipid homeostasis
C	0004758	molecular_function	serine C-palmitoyltransferase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0006665	biological_process	sphingolipid metabolic process
C	0009058	biological_process	biosynthetic process
C	0016020	cellular_component	membrane
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0017059	cellular_component	serine palmitoyltransferase complex
C	0030148	biological_process	sphingolipid biosynthetic process
C	0030170	molecular_function	pyridoxal phosphate binding
C	0046512	biological_process	sphingosine biosynthetic process
C	0046513	biological_process	ceramide biosynthetic process
C	0090156	biological_process	intracellular sphingolipid homeostasis
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0006666	biological_process	3-keto-sphinganine metabolic process
D	0008047	molecular_function	enzyme activator activity
D	0016020	cellular_component	membrane
D	0017059	cellular_component	serine palmitoyltransferase complex
D	0090154	biological_process	positive regulation of sphingolipid biosynthetic process
D	0090156	biological_process	intracellular sphingolipid homeostasis
E	0000139	cellular_component	Golgi membrane
E	0004438	molecular_function	phosphatidylinositol-3-phosphate phosphatase activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005741	cellular_component	mitochondrial outer membrane
E	0005783	cellular_component	endoplasmic reticulum
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005794	cellular_component	Golgi apparatus
E	0005797	cellular_component	Golgi medial cisterna
E	0016020	cellular_component	membrane
E	0016787	molecular_function	hydrolase activity
E	0016791	molecular_function	phosphatase activity
E	0017059	cellular_component	serine palmitoyltransferase complex
E	0032541	cellular_component	cortical endoplasmic reticulum
E	0034593	molecular_function	phosphatidylinositol bisphosphate phosphatase activity
E	0043812	molecular_function	phosphatidylinositol-4-phosphate phosphatase activity
E	0046856	biological_process	phosphatidylinositol dephosphorylation
E	0052629	molecular_function	phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity
E	0061909	biological_process	autophagosome-lysosome fusion
E	0072517	cellular_component	host cell viral assembly compartment
E	0090156	biological_process	intracellular sphingolipid homeostasis

Functional Information from PROSITE/UniProt

site_id	PS00599
Number of Residues	10
Details	AA_TRANSFER_CLASS_2 Aminotransferases class-II pyridoxal-phosphate attachment site. TFTKSFGAAG

Chain	Residue	Details
C	THR363-GLY372

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	571
Details	TOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:11792713

Chain	Residue	Details
E	MET1-TYR523
E	LYS574-ASP623
A	HIS206-SER222

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site_id	SWS_FT_FI2
Number of Residues	40
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
E	ILE524-PHE544
E	LYS553-PHE573
B	ALA425-LYS457
A	LEU185-THR205

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site_id	SWS_FT_FI3
Number of Residues	7
Details	TOPO_DOM: Lumenal => ECO:0000305|PubMed:11792713

Chain	Residue	Details
E	PRO545-SER552
B	PHE458-LYS558

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site_id	SWS_FT_FI4
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
E	LYS246
E	LYS358

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER32

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site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER56

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