8C7F
Crystal structure of beta-xylosidase mutant (D281N, E517Q) from Thermotoga maritima in complex with xylopentaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Xylosidase | polymer | 780 | 87106.0 | 2 | UniProt (Q9WXT1) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) In PDB | Thermotoga maritima MSB8 | |
| 2 | X | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 414.4 | 1 | In PDB BIRD (PRD_900117) GlyTouCan (G87429QT) | |||
| 3 | C | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 546.5 | 1 | In PDB GlyTouCan (G60650FG) | |||
| 4 | A, B | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 2 | Chemie (MPD) | |||
| 5 | water | water | 18.0 | 1322 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 778 (UniProt: Q9WXT1)
| PDB | External Database | Details |
|---|---|---|
| Asn 281 | Asp 281 | engineered mutation |
| Gln 517 | Glu 517 | engineered mutation |
| Ala 779 | - | expression tag |
| Ala 780 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 174212.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 960.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 236.3 | |
| All* | Total formula weight | 175409.2 |
