8C61

Structure of USP54 in complex with Lys63-linked diUbiquitin-PA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J	Inactive ubiquitin carboxyl-terminal hydrolase 54	polymer	350	39789.6	4	UniProt (Q70EL1) In PDB	Homo sapiens (human)	Inactive ubiquitin-specific peptidase 54
2	B, E, H, K	Polyubiquitin-B	polymer	75	8519.8	4	UniProt (P0CG47) In PDB	Homo sapiens (human)
3	C, F, I, L	Ubiquitin	polymer	76	8604.8	4	UniProt (P0CG47) In PDB	Homo sapiens (human)
4	J, A, D, G	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
5	G, J, A, D	prop-2-en-1-amine	non-polymer		57.1	4	Chemie (AYE)
6	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	G, C	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
8		water	water		18.0	213	Chemie (HOH)

Sequence modifications

A, D, G, J: 21 - 369 (UniProt: Q70EL1)

PDB	External Database	Details
Gly 20	-	expression tag

C, F, I, L: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Arg 63	Lys 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	227657.1
Non-Polymers*	Number of molecules	20
	Total formula weight	1130.2
All*	Total formula weight	228787.2

*Water molecules are not included.