8C61

Structure of USP54 in complex with Lys63-linked diUbiquitin-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	122.743, 126.559, 144.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.280 - 2.500
R-factor	0.2026
Rwork	0.200
R-free	0.24330
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.623
Data reduction software	DIALS (3.5.0)
Data scaling software	Aimless (1.12.10)
Phasing software	CRANK2 (2.0.281)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.280	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.062	1.239
Rmeas	0.066	1.313
Number of reflections	149985	7759
<I/σ(I)>	16.2	1.7
Completeness [%]	100.0
Redundancy	8.9
CC(1/2)	0.998	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	27.2 % (v/v) PEG 400, 0.1 M HEPES, 0.2 M calcium chloride