8C59
CpG specific M.MpeI methyltransferase crystallized in the presence of 5-bromocytosine (converted to 5mC) and 5-methylcytosine containing dsDNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Cytosine-specific methyltransferase | polymer | 395 | 46238.9 | 1 | UniProt (Q8EVR5) Pfam (PF00145) In PDB | Malacoplasma penetrans HF-2 | |
| 2 | B | DNA (5'-D(*CP*CP*AP*CP*AP*TP*GP*(5CM)P*GP*CP*TP*GP*AP*A)-3') | polymer | 14 | 4263.8 | 1 | synthetic construct | ||
| 3 | C | DNA (5'-D(*GP*TP*TP*CP*AP*GP*(5CM)P*GP*CP*AP*TP*GP*TP*G)-3') | polymer | 14 | 4325.8 | 1 | synthetic construct | ||
| 4 | A | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) | |||
| 5 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | A, B, C | GLYCEROL | non-polymer | 92.1 | 9 | Chemie (GOL) | |||
| 7 | A | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 8 | C | CARBONATE ION | non-polymer | 60.0 | 1 | Chemie (CO3) | |||
| 9 | water | water | 18.0 | 591 | Chemie (HOH) |
Sequence modifications
A: 1 - 395 (UniProt: Q8EVR5)
| PDB | External Database | Details |
|---|---|---|
| Arg 68 | Gln 68 | cloning artifact |
| Arg 71 | Lys 71 | cloning artifact |
| Pro 295 | Ser 295 | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 54828.6 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1502.4 | |
| All* | Total formula weight | 56331.0 |
