8C3N
Stapled peptide SP30 in complex with humanised RadA mutant HumRadA22
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA repair and recombination protein RadA | polymer | 231 | 25542.1 | 1 | UniProt (O74036) Pfam (PF08423) | Pyrococcus furiosus | Humanised RadA mutant HumRadA22 |
| 2 | B (B) | Breast cancer type 2 susceptibility protein | polymer | 29 | 2909.3 | 1 | UniProt (P51587) Pfam (PF00634) | Homo sapiens (human) | Fanconi anemia group D1 protein |
| 3 | C (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (B) | 4,6-diethylpyrimidin-2-amine | non-polymer | 151.2 | 1 | Chemie (RF6) | |||
| 6 | F, G (A, B) | water | water | 18.0 | 223 | Chemie (HOH) |
Sequence modifications
A: 107 - 349 (UniProt: O74036)
B: 2054 - 1243 (UniProt: P51587)
| PDB | External Database | Details |
|---|---|---|
| Met 107 | Arg 107 | engineered mutation |
| Ala 168 | Val 168 | engineered mutation |
| Met 169 | Ile 169 | engineered mutation |
| Tyr 170 | Trp 170 | engineered mutation |
| Leu 182 | Ile 182 | engineered mutation |
| Asp 198 | Lys 198 | engineered mutation |
| Asn 199 | His 199 | engineered mutation |
| Val 200 | Ile 200 | engineered mutation |
| Ala 201 | Tyr 201 | engineered mutation |
| Tyr 202 | Val 202 | engineered mutation |
| Gln 213 | Leu 213 | engineered mutation |
| Leu 215 | Val 215 | engineered mutation |
| Tyr 216 | Gln 216 | engineered mutation |
| Ser 219 | Glu 219 | engineered mutation |
| Ala 220 | Asp 220 | engineered mutation |
| Met 221 | Lys 221 | engineered mutation |
| Met 222 | Ile 222 | engineered mutation |
| Val 223 | Lys 223 | engineered mutation |
| Ser 225 | Leu 225 | engineered mutation |
| Tyr 232 | Val 232 | engineered mutation |
| Arg 263 | Lys 263 | engineered mutation |
| Phe 264 | His 264 | engineered mutation |
| Arg 266 | Ala 266 | engineered mutation |
| Met 267 | Asp 267 | engineered mutation |
| Glu 274 | Leu 274 | engineered mutation |
| Phe 275 | Tyr 275 | engineered mutation |
| Asn 300 | Arg 288 | engineered mutation |
| - | Pro 289 | deletion |
| - | Asp 290 | deletion |
| - | Ala 291 | deletion |
| - | Phe 292 | deletion |
| - | Phe 293 | deletion |
| - | Gly 294 | deletion |
| - | Asp 295 | deletion |
| - | Pro 296 | deletion |
| - | Thr 297 | deletion |
| - | Arg 298 | deletion |
| - | Pro 299 | deletion |
| - | Ile 300 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Cys 2055 | Phe 1216 | engineered mutation |
| Ser 2056 | Arg 1217 | engineered mutation |
| Ser 2059 | Tyr 1220 | engineered mutation |
| Thr 2060 | Ser 1221 | engineered mutation |
| Ser 2062 | His 1223 | engineered mutation |
| Lys 2064 | Thr 1225 | engineered mutation |
| Gln 1232 | Glu 1232 | engineered mutation |
| Cys 1234 | Leu 1234 | engineered mutation |
| Gly 1243 | Asp 1243 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 28451.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 602.7 | |
| All* | Total formula weight | 29054.1 |
