8C23

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (monoclinic form M200T#m)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, CCC, EEE, GGG	Isoaspartyl peptidase subunit alpha	polymer	178	19013.8	4	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli
2	BBB, DDD, FFF, HHH	Isoaspartyl peptidase subunit beta	polymer	143	14398.1	4	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli
3	AAA, CCC, EEE, GGG	SODIUM ION	non-polymer		23.0	6	Chemie (NA)
4	AAA, DDD, HHH	GLYCINE	non-polymer		75.1	3	Chemie (GLY)
5	AAA, BBB	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	BBB, DDD, EEE, GGG	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
7	CCC	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8		water	water		18.0	507	Chemie (HOH)

Sequence modifications

AAA, CCC, EEE, GGG: 2 - 178 (UniProt: P37595)

PDB	External Database	Details
Met 1	-	initiating methionine

BBB, DDD, FFF, HHH: 179 - 321 (UniProt: P37595)

PDB	External Database	Details
Thr 200	Met 200	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	133647.3
Non-Polymers*	Number of molecules	16
	Total formula weight	721.2
All*	Total formula weight	134368.5

*Water molecules are not included.