8C23
Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (monoclinic form M200T#m)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97630 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.461, 149.649, 105.130 |
Unit cell angles | 90.00, 126.72, 90.00 |
Refinement procedure
Resolution | 84.380 - 1.842 |
Rwork | 0.185 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.564 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.380 | 1.840 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.071 | |
Rmeas | 0.077 | |
Number of reflections | 133092 | 6430 |
<I/σ(I)> | 13.9 | |
Completeness [%] | 98.0 | |
Redundancy | 7 | |
CC(1/2) | 0.999 | 0.564 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-30% PEG4000,10-15% PEG 400,0.2M CaCl2 in 100 mM Tris-HCl pH 8.5, 100mM glycine |