8C23

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (monoclinic form M200T#m)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	C 1 2 1
Unit cell lengths	127.461, 149.649, 105.130
Unit cell angles	90.00, 126.72, 90.00

Refinement procedure

Resolution	84.380 - 1.842
Rwork	0.185
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.564
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.380	1.840
High resolution limit [Å]	1.840	1.840
Rmerge	0.071
Rmeas	0.077
Number of reflections	133092	6430
<I/σ(I)>	13.9
Completeness [%]	98.0
Redundancy	7
CC(1/2)	0.999	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20-30% PEG4000,10-15% PEG 400,0.2M CaCl2 in 100 mM Tris-HCl pH 8.5, 100mM glycine