8C0J

Structure of AmiB enzymatic domain bound to the EnvC LytM domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	N-acetylmuramoyl-L-alanine amidase	polymer	234	25585.7	2	UniProt (A0A482PQR2)	Citrobacter rodentium
2	B (B)	Murein hydrolase activator EnvC	polymer	149	16141.1	1	UniProt (A0A482PID1)	Citrobacter rodentium
3	D, F (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	E, G (A, C)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)

Sequence modifications

A, C: 190 - 422 (UniProt: A0A482PQR2)

PDB	External Database	Details
Met 189	-	initiating methionine

B: 293 - 427 (UniProt: A0A482PID1)

PDB	External Database	Details
Met 279	-	initiating methionine
Gly 280	-	expression tag
Ser 281	-	expression tag
Ser 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
Ser 289	-	expression tag
Gln 290	-	expression tag
Asp 291	-	expression tag
Pro 292	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67312.5
Non-Polymers*	Number of molecules	4
	Total formula weight	320.8
All*	Total formula weight	67633.3

*Water molecules are not included.