8C0J
Structure of AmiB enzymatic domain bound to the EnvC LytM domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 237.363, 237.363, 237.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.005 - 3.381 |
Rwork | 0.249 |
R-free | 0.29090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in silico model |
RMSD bond length | 0.008 |
RMSD bond angle | 1.405 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.340 | 3.650 |
High resolution limit [Å] | 3.380 | 3.380 |
Rpim | 0.088 | 0.686 |
Number of reflections | 16294 | 3305 |
<I/σ(I)> | 9.9 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 78.2 | |
CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Na Phosphate pH7.6 |