8C0I
Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200L (acyl-enzyme intermediate)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (AAA, CCC) | Isoaspartyl peptidase subunit alpha | polymer | 178 | 19013.8 | 2 | UniProt (P37595) Pfam (PF01112) | Escherichia coli | |
| 2 | B (BBB) | Isoaspartyl peptidase subunit beta | polymer | 143 | 14410.1 | 1 | UniProt (P37595) Pfam (PF01112) | Escherichia coli | |
| 3 | D (DDD) | Isoaspartyl peptidase subunit beta | polymer | 143 | 14525.2 | 1 | UniProt (P37595) Pfam (PF01112) | Escherichia coli | |
| 4 | E, H (AAA, CCC) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | F, G (AAA) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | I (DDD) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | J, K, L, M (AAA, BBB, CCC, DDD) | water | water | 18.0 | 185 | Chemie (HOH) |
Sequence modifications
AAA, CCC: 2 - 178 (UniProt: P37595)
BBB: 179 - 321 (UniProt: P37595)
DDD: 179 - 321 (UniProt: P37595)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| PDB | External Database | Details |
|---|---|---|
| Leu 200 | Met 200 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Aei 179 | Thr 179 | modified residue |
| Leu 200 | Met 200 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 66962.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 130.0 | |
| All* | Total formula weight | 67092.9 |
