8C0I

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200L (acyl-enzyme intermediate)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, CCC	Isoaspartyl peptidase subunit alpha	polymer	178	19013.8	2	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli
2	BBB	Isoaspartyl peptidase subunit beta	polymer	143	14410.1	1	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli
3	DDD	Isoaspartyl peptidase subunit beta	polymer	143	14525.2	1	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli
4	AAA, CCC	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	AAA	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	DDD	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	185	Chemie (HOH)

Sequence modifications

AAA, CCC: 2 - 178 (UniProt: P37595)

PDB	External Database	Details
Met 1	-	initiating methionine

BBB: 179 - 321 (UniProt: P37595)

PDB	External Database	Details
Leu 200	Met 200	engineered mutation

DDD: 179 - 321 (UniProt: P37595)

PDB	External Database	Details
Aei 179	Thr 179	modified residue
Leu 200	Met 200	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66962.9
Non-Polymers*	Number of molecules	5
	Total formula weight	130.0
All*	Total formula weight	67092.9

*Water molecules are not included.