8C0I
Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200L (acyl-enzyme intermediate)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU PhotonJet-S |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-31 |
Detector | RIGAKU HyPix-3000 |
Wavelength(s) | 1.54184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.710, 74.851, 146.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.300 - 1.900 |
Rwork | 0.232 |
R-free | 0.27520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.553 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.300 | 1.900 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.148 | 0.283 |
Rmeas | 0.177 | 0.344 |
Number of reflections | 42775 | 2856 |
<I/σ(I)> | 18 | 3.2 |
Completeness [%] | 97.6 | 97.8 |
Redundancy | 3.1 | |
CC(1/2) | 0.949 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 PEG4000, 10% PEG400, 0.2 M MgCl2 in 100 mM Tris-HCl pH 8.5 |