8BWL

Crystal structure of human Twisted gastrulation protein homolog 1 (TWSG1) in complex with human Growth Differentiation factor 5 (GDF5) and calcium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Growth/differentiation factor 5	polymer	121	13729.8	2	UniProt (P43026) In PDB	Homo sapiens (human)	GDF-5,Bone morphogenetic protein 14,BMP-14,Cartilage-derived morphogenetic protein 1,CDMP-1,Lipopolysaccharide-associated protein 4,LAP-4,LPS-associated protein 4,Radotermin
2	C, D	Twisted gastrulation protein homolog 1	polymer	69	7499.7	2	UniProt (Q9GZX9) In PDB	Homo sapiens (human)
3	A, B, C, D	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4		water	water		18.0	150	Chemie (HOH)

Sequence modifications

A, B: 382 - 501 (UniProt: P43026)

PDB	External Database	Details
Met 381	-	initiating methionine

C, D: 26 - 83 (UniProt: Q9GZX9)

PDB	External Database	Details
Glu 23	-	expression tag
Thr 24	-	expression tag
Gly 25	-	expression tag
Gly 84	-	expression tag
Thr 85	-	expression tag
Leu 86	-	expression tag
Glu 87	-	expression tag
Val 88	-	expression tag
Leu 89	-	expression tag
Phe 90	-	expression tag
Gln 91	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	42459.1
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	42619.5

*Water molecules are not included.