8BV5

Focus refinement of soluble domain of Adenylyl cyclase 8 bound to stimulatory G protein, Forskolin, ATPalphaS, and Ca2+/Calmodulin in lipid nanodisc conditions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Adenylate cyclase type 8	polymer	1253	140192.3	1	UniProt (E1BQ12) Pfam (PF16214) Pfam (PF00211) Pfam (PF06327) In PDB	Bos taurus (cattle)
2	B	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	405	47003.8	1	UniProt (P04896) Pfam (PF00503) In PDB	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
3	A	FORSKOLIN	non-polymer		410.5	1	Chemie (FOK)
4	B	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	1	Chemie (GSP)
5	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

B: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Gly 65	-	insertion
Asn 65	Gln 78	conflict
Lys 65	Arg 81	conflict
Gly 395	-	expression tag
Gly 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	187196.1
Non-Polymers*	Number of molecules	3
	Total formula weight	974.1
All*	Total formula weight	188170.2

*Water molecules are not included.