8BRV
Escherichia coli methionyl-tRNA synthetase mutant L13M,I297C complexed with beta3-methionine.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Methionine--tRNA ligase | polymer | 568 | 64738.0 | 1 | UniProt (P00959) Pfam (PF09334) Pfam (PF19303) | Escherichia coli | Methionyl-tRNA synthetase,MetRS |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D (A) | CITRIC ACID | non-polymer | 192.1 | 2 | Chemie (CIT) | |||
| 4 | E (A) | (3R)-3-amino-5-(methylsulfanyl)pentanoic acid | non-polymer | 163.2 | 1 | Chemie (B3M) | |||
| 5 | F (A) | water | water | 18.0 | 694 | Chemie (HOH) |
Sequence modifications
A: 1 - 547 (UniProt: P00959)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Met 13 | Leu 14 | engineered mutation |
| Cys 297 | Ile 298 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 64738.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 612.9 | |
| All* | Total formula weight | 65350.9 |
