8BRV
Escherichia coli methionyl-tRNA synthetase mutant L13M,I297C complexed with beta3-methionine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.650, 45.470, 86.470 |
Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
Resolution | 48.680 - 1.530 |
R-factor | 0.1528 |
Rwork | 0.151 |
R-free | 0.19270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.965 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmeas | 0.109 | 1.289 |
Number of reflections | 86940 | 13250 |
<I/σ(I)> | 12.44 | 1.25 |
Completeness [%] | 98.9 | 93.7 |
Redundancy | 6.7 | 6.5 |
CC(1/2) | 0.998 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.08M ammonium citrate, 30 mM potassium phosphate |