8BKF

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (crystal M200T#o)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, CCC	Isoaspartyl peptidase subunit alpha	polymer	178	19013.8	2	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli K-12
2	BBB, DDD	Isoaspartyl peptidase subunit beta	polymer	143	14398.1	2	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli K-12
3	AAA, CCC	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	BBB, DDD	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5	BBB	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6		water	water		18.0	594	Chemie (HOH)

Sequence modifications

AAA, CCC: 2 - 178 (UniProt: P37595)

PDB	External Database	Details
Met 1	-	initiating methionine

BBB, DDD: 179 - 321 (UniProt: P37595)

PDB	External Database	Details
Thr 200	Met 200	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66823.7
Non-Polymers*	Number of molecules	7
	Total formula weight	212.1
All*	Total formula weight	67035.8

*Water molecules are not included.