8BKF

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (crystal M200T#o)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976300
Spacegroup name	P 21 21 21
Unit cell lengths	49.852, 75.886, 147.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.890 - 1.221
Rwork	0.128
R-free	0.15020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.006
RMSD bond angle	1.295
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.890	1.240
High resolution limit [Å]	1.220	1.220
Rmerge	0.072	1.696
Rmeas	0.075	1.771
Number of reflections	166404	8052
<I/σ(I)>	16.4	1.6
Completeness [%]	99.9	98.6
Redundancy	12.7	12.1
CC(1/2)	0.999	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20-30% PEG4000,10-15% PEG400, 0,2 M magnesium chloride in 100mM Tris-HCl pH 8.5