8BJH
chimera of the inactive ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, with the double mutation K3528M and K3535I, fused to a proline-Rich-Domain (PRD) and profilin, bound to Latrunculin B-ADP-Mg-actin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Actin, alpha skeletal muscle, intermediate form | polymer | 375 | 41862.6 | 1 | UniProt (P68135) Pfam (PF00022) | Oryctolagus cuniculus (rabbit) | |
| 2 | B (B) | Putative Adenylate cyclase,Profilin-1 | polymer | 591 | 65218.9 | 1 | UniProt (A0A6N3LUE9) UniProt (P07737) Pfam (PF00235) UniProt (by SIFTS) (A0A9P1NJI6) | Vibrio nigripulchritudo | MARTX toxin,Epididymis tissue protein Li 184a,Profilin I |
| 3 | C (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 5 | E (A) | LATRUNCULIN B | non-polymer | 395.5 | 1 | Chemie (LAB) PubChem (16760044) PubChem (20054954) PubChem (3892) PubChem (22832299) PubChem (23424960) PubChem (23863831) PubChem (25215545) PubChem (72940) PubChem (57370013) PubChem (59169653) PubChem (59431496) PubChem (67441199) PubChem (67441200) PubChem (56928133) PubChem (88895461) PubChem (90912141) PubChem (5352046) PubChem (5353712) PubChem (98044135) PubChem (92209860) PubChem (6436219) PubChem (98044136) PubChem (98044137) PubChem (10000783) PubChem (14504487) PubChem (162928966) PubChem (129388766) PubChem (129388767) PubChem (146159701) PubChem (154825202) PubChem (162928961) PubChem (162928962) PubChem (162928963) PubChem (162928964) PubChem (162928965) PubChem (162928967) PubChem (163195209) PubChem (168317464) PubChem (171039486) | |||
| 6 | F, O (A, B) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) PubChem (3777159) | |||
| 7 | G, P, Q (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) PubChem (753) | |||
| 8 | H, I, J, K, L... (A, B) | HYDROGEN PEROXIDE | non-polymer | 34.0 | 8 | Chemie (PEO) PubChem (784) | |||
| 9 | M, N (B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 10 | R (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) PubChem (8117) | |||
| 11 | V, W (A, B) | water | water | 18.0 | 958 | Chemie (HOH) |
Sequence modifications
B: 455 - 896 (UniProt: A0A6N3LUE9)
B: 903 - 1041 (UniProt: P07737)
| PDB | External Database | Details |
|---|---|---|
| Gly 451 | - | expression tag |
| Pro 452 | - | expression tag |
| Gly 453 | - | expression tag |
| Ser 454 | - | expression tag |
| Met 528 | Lys 3528 | engineered mutation |
| Ile 535 | Lys 3535 | engineered mutation |
| Pro 897 | - | linker |
| Pro 898 | - | linker |
| Pro 899 | - | linker |
| Pro 900 | - | linker |
| Gly 901 | - | linker |
| Gly 902 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 107081.5 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 1938.0 | |
| All* | Total formula weight | 109019.4 |
