8BJH
chimera of the inactive ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, with the double mutation K3528M and K3535I, fused to a proline-Rich-Domain (PRD) and profilin, bound to Latrunculin B-ADP-Mg-actin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Actin, alpha skeletal muscle, intermediate form | polymer | 375 | 41862.6 | 1 | UniProt (P68135) Pfam (PF00022) | Oryctolagus cuniculus (rabbit) | |
| 2 | B (B) | Putative Adenylate cyclase,Profilin-1 | polymer | 591 | 65218.9 | 1 | UniProt (A0A6N3LUE9) UniProt (P07737) Pfam (PF03497) Pfam (PF00235) UniProt (by SIFTS) (A0A9P1NJI6) | Vibrio nigripulchritudo | MARTX toxin,Epididymis tissue protein Li 184a,Profilin I |
| 3 | C (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (A) | LATRUNCULIN B | non-polymer | 395.5 | 1 | Chemie (LAB) | |||
| 6 | F, O (A, B) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 7 | G, P, Q (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 8 | H, I, J, K, L... (A, B) | HYDROGEN PEROXIDE | non-polymer | 34.0 | 8 | Chemie (PEO) | |||
| 9 | M, N (B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 10 | R (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 11 | V, W (A, B) | water | water | 18.0 | 958 | Chemie (HOH) |
Sequence modifications
B: 455 - 896 (UniProt: A0A6N3LUE9)
B: 903 - 1041 (UniProt: P07737)
| PDB | External Database | Details |
|---|---|---|
| Gly 451 | - | expression tag |
| Pro 452 | - | expression tag |
| Gly 453 | - | expression tag |
| Ser 454 | - | expression tag |
| Met 528 | Lys 3528 | engineered mutation |
| Ile 535 | Lys 3535 | engineered mutation |
| Pro 897 | - | linker |
| Pro 898 | - | linker |
| Pro 899 | - | linker |
| Pro 900 | - | linker |
| Gly 901 | - | linker |
| Gly 902 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 107081.5 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 1938.0 | |
| All* | Total formula weight | 109019.4 |
