8BHG

GABA-A receptor a5 heteromer - a5V2 - Bretazenil

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E (A, C, D, E)	Gamma-aminobutyric acid receptor subunit alpha-5	polymer	350	40148.9	4	UniProt (P31644) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-5
2	B (B)	Gamma-aminobutyric acid receptor subunit gamma-2	polymer	379	43751.9	1	UniProt (P18507) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit gamma-2
3	F, J, K, S, T (A, B, D)	Bretazenil	non-polymer		418.3	5	Chemie (QMU)
4	G, L, P, U, W (A, B, C, D, E)	DECYL-BETA-D-MALTOPYRANOSIDE	non-polymer		482.6	5	Chemie (DMU)
5	H, M, Q (A, B, C)	NITRATE ION	non-polymer		62.0	3	Chemie (NO3)
6	I, N, O, R, V... (A, B, C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
7	AA, BA, CA, Y, Z (C, D, E, A, B)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

A, C, D, E: 1 - 457 (UniProt: P31644)

PDB	External Database	Details
Met 48	Ile 79	engineered mutation
Asn 51	Thr 82	engineered mutation
Ile 67	Val 98	engineered mutation
Ala 70	Arg 101	engineered mutation
Thr 72	Ser 103	engineered mutation
Asp 90	Asn 121	engineered mutation
Arg 92	Leu 123	engineered mutation
Val 93	Leu 124	engineered mutation
Asp 95	Ser 126	engineered mutation
Gln 96	Lys 127	engineered mutation
Asp 107	Gly 138	engineered mutation
Phe 111	Ile 142	engineered mutation
Gly 114	Asn 145	conflict
Met 121	Leu 152	engineered mutation
Ile 124	Leu 155	engineered mutation
Trp 125	Glu 156	engineered mutation
Asn 126	Asp 157	engineered mutation
Arg 129	Thr 160	engineered mutation
Val 130	Leu 161	engineered mutation
Asp 145	Gln 176	engineered mutation
Glu 153	Ala 184	engineered mutation
Gln 154	His 185	engineered mutation
Asn 155	Ala 186	engineered mutation
Ala 256	Pro 287	engineered mutation
Leu 305	Ile 336	engineered mutation
Phe 309	Thr 340	engineered mutation
Ile 313	Phe 344	engineered mutation
Ser 316	Arg 347	conflict
Gln 317	Gly 348	conflict
Pro 318	Trp 349	conflict
-	Trp 351	deletion
-	Asp 352	deletion
-	Gly 353	deletion
-	Lys 354	deletion
-	Lys 355	deletion
-	Ala 356	deletion
-	Leu 357	deletion
-	Glu 358	deletion
-	Ala 359	deletion
-	Ala 360	deletion
-	Lys 361	deletion
-	Ile 362	deletion
-	Lys 363	deletion
-	Lys 364	deletion
-	Lys 365	deletion
-	Arg 366	deletion
-	Glu 367	deletion
-	Val 368	deletion
-	Ile 369	deletion
-	Leu 370	deletion
-	Asn 371	deletion
-	Lys 372	deletion
-	Ser 373	deletion
-	Thr 374	deletion
-	Asn 375	deletion
-	Ala 376	deletion
-	Phe 377	deletion
-	Thr 378	deletion
-	Thr 379	deletion
-	Gly 380	deletion
-	Lys 381	deletion
-	Met 382	deletion
-	Ser 383	deletion
-	His 384	deletion
-	Pro 385	deletion
-	Pro 386	deletion
-	Asn 387	deletion
-	Ile 388	deletion
-	Pro 389	deletion
-	Lys 390	deletion
-	Glu 391	deletion
-	Gln 392	deletion
-	Thr 393	deletion
-	Pro 394	deletion
-	Ala 395	deletion
-	Gly 396	deletion
-	Thr 397	deletion
-	Ser 398	deletion
-	Asn 399	deletion
-	Thr 400	deletion
-	Thr 401	deletion
-	Ser 402	deletion
-	Val 403	deletion
-	Ser 404	deletion
-	Val 405	deletion
-	Lys 406	deletion
-	Pro 407	deletion
-	Ser 408	deletion
-	Glu 409	deletion
-	Glu 410	deletion
-	Lys 411	deletion
-	Thr 412	deletion
-	Ser 413	deletion
-	Glu 414	deletion
-	Ser 415	deletion
-	Lys 416	deletion
-	Lys 417	deletion
-	Thr 418	deletion
-	Tyr 419	deletion
-	Asn 420	deletion
Arg 320	Ser 421	conflict
Ala 321	Ile 422	conflict
Ala 322	Ser 423	conflict
Ile 441	Val 435	conflict

B: 1 - 486 (UniProt: P18507)

PDB	External Database	Details
Thr 11	Ala 50	conflict
Ile 233	Met 272	conflict
Val 237	Thr 276	conflict
Leu 242	Ile 281	conflict
Ile 245	Thr 284	conflict
Met 246	Leu 285	conflict
Thr 247	Ile 286	conflict
Ile 249	Val 288	conflict
Gln 252	Trp 291	conflict
Leu 257	Ile 296	conflict
Arg 259	Lys 298	conflict
Glu 260	Asp 299	conflict
Ser 261	Ala 300	conflict
Ala 263	Pro 302	conflict
Val 267	Ser 306	conflict
Phe 268	Leu 307	conflict
Val 270	Ile 309	conflict
Ile 281	Thr 320	conflict
Ser 282	Ile 321	conflict
Asn 285	Lys 324	conflict
Ala 291	Ser 330	conflict
Ala 293	Val 332	conflict
Trp 298	Leu 337	conflict
Ile 300	Val 339	conflict
Ala 301	Ser 340	conflict
Tyr 304	Phe 343	conflict
Ala 305	Ile 344	conflict
Ile 312	Val 351	conflict
Phe 314	Tyr 353	conflict
Ala 315	Gly 354	conflict
Val 317	Leu 356	conflict
Asn 318	His 357	conflict
Thr 321	-	insertion
Lys 322	Val 360	conflict
Gln 324	-	insertion
Pro 325	-	insertion
Ala 326	Asn 362	conflict
Ala 328	Lys 364	conflict
Ala 329	Pro 365	conflict
Lys 330	Ser 366	conflict
Ile 438	Lys 367	conflict
Arg 440	Lys 369	conflict
Leu 441	Asp 370	conflict
Ser 442	Lys 371	conflict
Arg 443	Lys 372	conflict
Ile 444	Lys 373	conflict
Ala 445	Lys 374	conflict
Phe 446	Asn 375	conflict
Phe 450	-	insertion
Gly 451	Arg 379	conflict
Ile 452	Met 380	conflict
Asn 454	-	insertion
Leu 455	Ser 382	conflict
Val 456	Phe 383	conflict
Tyr 457	Lys 384	conflict
Trp 458	Ala 385	conflict
Ala 459	Pro 386	conflict
Tyr 461	Ile 388	conflict
Leu 462	Asp 389	conflict
Asn 463	Ile 390	conflict
Glu 465	-	insertion
Gln 467	-	insertion
Leu 468	-	insertion
Lys 469	Arg 393	conflict
Ala 470	Ser 394	conflict
Pro 471	Ala 395	conflict
Pro 473	-	insertion
His 474	Ile 397	conflict
Gly 476	Met 399	conflict
Thr 477	Asn 400	conflict
Thr 478	Asn 401	conflict
Glu 479	Ala 402	conflict
Ser 481	His 404	conflict
Gln 482	Leu 405	conflict
Val 483	Gln 406	conflict
Ala 484	Glu 407	conflict
Pro 485	Arg 408	conflict
Ala 486	Asp 409	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	204347.4
Non-Polymers*	Number of molecules	19
	Total formula weight	6017.5
All*	Total formula weight	210364.9

*Water molecules are not included.