8BEO
Crystal structure of E. coli glyoxylate carboligase mutant I393A with MAP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Glyoxylate carboligase | polymer | 616 | 67237.2 | 6 | UniProt (P0AEP7) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) | Escherichia coli K-12 | Tartronate-semialdehyde synthase |
| 2 | AB, DA, EA, EB, FA... (C, A, B, D, E...) | MAGNESIUM ION | non-polymer | 24.3 | 13 | Chemie (MG) | |||
| 3 | BB, GA, I, OB, QC... (C, B, A, D, F...) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 6 | Chemie (FAD) | |||
| 4 | AC, CB, HA, J, PB... (E, C, B, A, D...) | 3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-2-{(1S)-1-HYDROXY-1-[(R)-HYDROXY(METHOXY)PHOSPHORYL]ETHYL}-5-(2-{[(S)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM | non-polymer | 563.4 | 6 | Chemie (TDK) | |||
| 5 | FB, IA, K, RB (C, B, A, D) | METHYL HYDROGEN (S)-ACETYLPHOSPHONATE | non-polymer | 138.1 | 4 | Chemie (ALU) | |||
| 6 | BC, DB, JA, L, QB... (E, C, B, A, D...) | 2,3-DIMETHOXY-5-METHYL-1,4-BENZOQUINONE | non-polymer | 182.2 | 6 | Chemie (UQ0) | |||
| 7 | AA, GB, M, N, NA... (A, C, B, F) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 8 | Chemie (PEG) | |||
| 8 | KA, O, P (B, A) | 2,3-DIHYDROXY-1,4-DITHIOBUTANE | non-polymer | 154.3 | 3 | Chemie (DTT) | |||
| 9 | AD, CA, DC, EC, FC... (F, A, E, C, B...) | FORMIC ACID | non-polymer | 46.0 | 35 | Chemie (FMT) | |||
| 10 | S (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 11 | BA, IC, JB, KC, LC... (A, E, C, B, D...) | SODIUM ION | non-polymer | 23.0 | 15 | Chemie (NA) | |||
| 12 | CC (E) | (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL | non-polymer | 154.3 | 1 | Chemie (DTU) | |||
| 13 | HC (E) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 14 | BD, CD, DD, ED, FD... (A, B, C, D, E...) | water | water | 18.0 | 2119 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 593 (UniProt: P0AEP7)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Ser -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| His -3 | - | expression tag |
| Met -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 393 | Ile 393 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 403423.5 | |
| Non-Polymers* | Number of molecules | 99 |
| Total formula weight | 13662.1 | |
| All* | Total formula weight | 417085.5 |
