8BEF
Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CI membrane core)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 119 | 13969.4 | 1 | UniProt (P92533) Pfam (PF00507) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 3 |
| 2 | B (H) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 325 | 36020.1 | 1 | UniProt (B5TM92) Pfam (PF00146) UniProt (by SIFTS) (P92558) | Arabidopsis thaliana (thale cress) | |
| 3 | C (J) | NADH-ubiquinone oxidoreductase chain 6 | polymer | 205 | 23690.4 | 1 | UniProt (A0A2P2CLG1) Pfam (PF00499) UniProt (by SIFTS) (P60497) | Arabidopsis thaliana (thale cress) | |
| 4 | D (K) | NADH-ubiquinone oxidoreductase chain 4L | polymer | 100 | 11193.6 | 1 | UniProt (Q04614) Pfam (PF00420) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 4L |
| 5 | E (L) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 669 | 74498.0 | 1 | UniProt (B5TM94) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) UniProt (by SIFTS) (P29388) | Arabidopsis thaliana (thale cress) | |
| 6 | F (M) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 495 | 55995.7 | 1 | UniProt (P93313) Pfam (PF00361) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 4 |
| 7 | G (N) | NADH-ubiquinone oxidoreductase chain 2 | polymer | 499 | 55486.8 | 1 | UniProt (O05000) Pfam (PF00361) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 2 |
| 8 | H (O) | AT3G07480.1 | polymer | 159 | 17626.2 | 1 | UniProt (A0A384LA38) UniProt (by SIFTS) (Q9SRR8) | Arabidopsis thaliana (thale cress) | |
| 9 | I (X) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B | polymer | 106 | 11986.0 | 1 | UniProt (Q8LGE7) Pfam (PF06747) | Arabidopsis thaliana (thale cress) | |
| 10 | J (Y) | Outer envelope pore protein 16-3, chloroplastic/mitochondrial | polymer | 159 | 17017.3 | 1 | UniProt (O48528) Pfam (PF02466) | Arabidopsis thaliana (thale cress) | Chloroplastic outer envelope pore protein of 16 kDa 3,AtOEP16-3,OEP16-3,Mitochondrial complex I subunit B14.7 |
| 11 | K (Z) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A | polymer | 143 | 16145.6 | 1 | UniProt (Q8RWA7) Pfam (PF06212) | Arabidopsis thaliana (thale cress) | Protein MATERNAL EFFECT EMBRYO ARREST 4 |
| 12 | L (a) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 | polymer | 65 | 7349.6 | 1 | UniProt (Q9C9Z5) Pfam (PF15879) | Arabidopsis thaliana (thale cress) | |
| 13 | M (b) | At2g46540/F11C10.23 | polymer | 65 | 6810.2 | 1 | UniProt (Q9ZPY5) | Arabidopsis thaliana (thale cress) | Expressed protein,F11C10.23/F11C10.23,Fiber |
| 14 | N (d) | Excitatory amino acid transporter | polymer | 81 | 9220.7 | 1 | UniProt (Q94AL6) | Arabidopsis thaliana (thale cress) | |
| 15 | O (e) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B | polymer | 83 | 9914.1 | 1 | UniProt (Q9LZI6) Pfam (PF10200) | Arabidopsis thaliana (thale cress) | |
| 16 | P (f) | At4g16450 | polymer | 106 | 11383.0 | 1 | UniProt (Q84W12) Pfam (PF10785) | Arabidopsis thaliana (thale cress) | NADH-ubiquinone oxidoreductase,Uncharacterized protein At4g16450 |
| 17 | Q (i) | P1 | polymer | 98 | 11808.2 | 1 | UniProt (A0A178W1I8) Pfam (PF17250) UniProt (by SIFTS) (Q9FYF8) | Arabidopsis thaliana (thale cress) | |
| 18 | R (u) | Uncharacterized protein At1g67785 | polymer | 63 | 7546.8 | 1 | UniProt (Q8VZ65) | Arabidopsis thaliana (thale cress) | |
| 19 | S (v) | Uncharacterized protein At2g27730, mitochondrial | polymer | 113 | 11965.5 | 1 | UniProt (Q9ZUX4) | Arabidopsis thaliana (thale cress) | |
| 20 | T (x) | Gamma carbonic anhydrase-like 2, mitochondrial | polymer | 256 | 27985.1 | 1 | UniProt (Q9SMN1) | Arabidopsis thaliana (thale cress) | GAMMA CAL2 |
| 21 | U (y) | Gamma carbonic anhydrase 2, mitochondrial | polymer | 278 | 30102.2 | 1 | UniProt (Q9C6B3) Pfam (PF00132) | Arabidopsis thaliana (thale cress) | GAMMA CA2,Transcription factor APFI |
| 22 | V (z) | Gamma carbonic anhydrase 1, mitochondrial | polymer | 275 | 30010.0 | 1 | UniProt (Q9FWR5) | Arabidopsis thaliana (thale cress) | GAMMA CA1 |
| 23 | BA, CA, EA, W, ZA (L, M, A, y) | (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE | non-polymer | 751.0 | 5 | Chemie (PGT) | |||
| 24 | X (H) | Ubiquinone-9 | non-polymer | 795.2 | 1 | Chemie (UQ9) | |||
| 25 | AB, DA, FA, GA, IA... (z, L, M, N, Y...) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 10 | Chemie (PTY) | |||
| 26 | AA, HA, PA, Z (K, M, a, H) | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside | non-polymer | 1165.3 | 4 | Chemie (Q7G) | |||
| 27 | MA (O) | FE (III) ION | non-polymer | 55.8 | 1 | Chemie (FE) | |||
| 28 | OA (Y) | 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE | non-polymer | 522.8 | 1 | Chemie (UQ5) | |||
| 29 | QA, TA, WA (d, f, v) | (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE | non-polymer | 763.1 | 3 | Chemie (PC7) | |||
| 30 | RA, SA (f) | 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE | non-polymer | 705.0 | 2 | Chemie (3PH) | |||
| 31 | UA, VA (u) | CARDIOLIPIN | non-polymer | 1464.0 | 2 | Chemie (CDL) | |||
| 32 | XA (y) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 33 | YA (y) | Butyryl Coenzyme A | non-polymer | 837.6 | 1 | Chemie (BCO) | |||
| 34 | BB, CB, DB, EB, FB... (A, H, J, K, L...) | water | water | 18.0 | 1284 | Chemie (HOH) |
Sequence modifications
K: 1 - 100 (UniProt: Q04614)
L: 1 - 669 (UniProt: B5TM94)
M: 1 - 495 (UniProt: P93313)
| PDB | External Database | Details |
|---|---|---|
| Leu 44 | Ser 44 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Phe 91 | Ser 91 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Phe 146 | Pro 146 | variant |
| Leu 326 | Pro 326 | variant |
| Phe 383 | Ser 383 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 22 |
| Total formula weight | 497724.6 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 24661.0 | |
| All* | Total formula weight | 522385.6 |
