8BE4

Crystal structure of SOS1-KRasG12V-Nanobody14

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (S)	Son of sevenless homolog 1	polymer	507	59348.8	1	UniProt (Q07889) Pfam (PF00618) Pfam (PF00617)	Homo sapiens (human)	SOS-1
2	B (R)	Isoform 2B of GTPase KRas	polymer	190	21675.3	1	UniProt (P01116-2) Pfam (PF00071) UniProt (by SIFTS) (P01116)	Homo sapiens (human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
3	C (C000)	Nanobody14	polymer	128	14070.6	1		Lama glama
4	D, E, F (S, R, C000)	water	water		18.0	551	Chemie (HOH)

Sequence modifications

S: 564 - 1049 (UniProt: Q07889)

PDB	External Database	Details
Met 543	-	initiating methionine
Gly 544	-	expression tag
Ser 545	-	expression tag
Ser 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag
Ser 553	-	expression tag
Ser 554	-	expression tag
Gly 555	-	expression tag
Leu 556	-	expression tag
Val 557	-	expression tag
Pro 558	-	expression tag
Arg 559	-	expression tag
Gly 560	-	expression tag
Ser 561	-	expression tag
His 562	-	expression tag
Met 563	-	expression tag

R: 1 - 169 (UniProt: P01116-2)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95094.7
All*	Total formula weight	95094.7

*Water molecules are not included.