8BE3

Crystal structure of KRasG12V-Nanobody84

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (AA0A, A00A)	Nanobody84	polymer	122	13519.1	2		Lama glama
2	B, C (R, A)	Isoform 2B of GTPase KRas	polymer	190	21675.3	2	UniProt (P01116-2) Pfam (PF00071) UniProt (by SIFTS) (P01116)	Homo sapiens (human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
3	E, G (R, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
4	F, H (R, A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
5	I, J, K, L (AA0A, R, A, A00A)	water	water		18.0	350	Chemie (HOH)

Sequence modifications

R, A: 1 - 169 (UniProt: P01116-2)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70388.7
Non-Polymers*	Number of molecules	4
	Total formula weight	935.0
All*	Total formula weight	71323.8

*Water molecules are not included.