8BE3

Crystal structure of KRasG12V-Nanobody84

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (AA0A, A00A)	Nanobody84	polymer	122	13519.1	2		Lama glama
2	B, C (R, A)	Isoform 2B of GTPase KRas	polymer	190	21675.3	2	UniProt (P01116-2) Pfam (PF00071) UniProt (by SIFTS) (P01116)	Homo sapiens (human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
3	E, G (R, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, H (R, A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
5	I, J, K, L (AA0A, R, A, A00A)	water	water		18.0	350	Chemie (HOH)

Sequence modifications

R, A: 1 - 169 (UniProt: P01116-2)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70388.7
Non-Polymers*	Number of molecules	4
	Total formula weight	935.0
All*	Total formula weight	71323.8

*Water molecules are not included.