8BE2

Crystal structure of SOS1-Nanobody77

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (S)	Son of sevenless homolog 1	polymer	507	59348.8	1	UniProt (Q07889) Pfam (PF00618) Pfam (PF00617)	Homo sapiens (human)	SOS-1
2	B (N)	Nanobody 77	polymer	127	13964.4	1		Lama glama
3	C, D, E, F, G (S, N)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	H, I (S, N)	water	water		18.0	409	Chemie (HOH)

Sequence modifications

S: 564 - 1049 (UniProt: Q07889)

PDB	External Database	Details
Met 543	-	initiating methionine
Gly 544	-	expression tag
Ser 545	-	expression tag
Ser 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag
Ser 553	-	expression tag
Ser 554	-	expression tag
Gly 555	-	expression tag
Leu 556	-	expression tag
Val 557	-	expression tag
Pro 558	-	expression tag
Arg 559	-	expression tag
Gly 560	-	expression tag
Ser 561	-	expression tag
His 562	-	expression tag
Met 563	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73313.2
Non-Polymers*	Number of molecules	5
	Total formula weight	480.3
All*	Total formula weight	73793.6

*Water molecules are not included.