8BAM

Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, tenfold mutant active on propanol syringol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Probable vanillyl-alcohol oxidase	polymer	526	58872.6	2	UniProt (Q0SBK1) Pfam (PF01565) Pfam (PF02913) In PDB	Rhodococcus jostii RHA1
2	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	A, B	2,6-dimethoxy-4-(3-oxidanylpropyl)phenol	non-polymer		212.2	2	Chemie (QBF)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	703	Chemie (HOH)

Sequence modifications

A, B: 1 - 526 (UniProt: Q0SBK1)

PDB	External Database	Details
His 81	Ser 81	engineered mutation
Glu 151	Asp 151	engineered mutation
Gln 381	Leu 381	engineered mutation
Val 394	Ser 394	engineered mutation
Met 423	Ala 423	engineered mutation
Ser 425	Gln 425	engineered mutation
Val 427	Ile 427	engineered mutation
Tyr 434	His 434	engineered mutation
Asp 445	Ile 445	engineered mutation
Pro 518	Ser 518	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	117745.1
Non-Polymers*	Number of molecules	6
	Total formula weight	2179.8
All*	Total formula weight	119924.9

*Water molecules are not included.