8BAM
Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, tenfold mutant active on propanol syringol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-29 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.965459 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.654, 98.058, 177.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.700 - 1.650 |
R-factor | 0.1741 |
Rwork | 0.172 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fxd |
RMSD bond length | 0.007 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.700 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.093 | 1.023 |
Number of reflections | 120308 | 5385 |
<I/σ(I)> | 12.8 | 1.7 |
Completeness [%] | 99.5 | 91.1 |
Redundancy | 6.4 | 5.9 |
CC(1/2) | 0.998 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M TrizmaHCl pH 8.0, 25% PEG6000 |