8B9D

Human replisome bound by Pol Alpha

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	DNA replication licensing factor MCM2	polymer	904	102034.1	1	UniProt (P49736) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	Minichromosome maintenance protein 2 homolog,Nuclear protein BM28
2	3	DNA replication licensing factor MCM3	polymer	808	91110.9	1	UniProt (P25205) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102
3	4	DNA replication licensing factor MCM4	polymer	863	96684.9	1	UniProt (P33991) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	CDC21 homolog,P1-CDC21
4	5	DNA replication licensing factor MCM5	polymer	734	82406.6	1	UniProt (P33992) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) In PDB	Homo sapiens (human)	CDC46 homolog,P1-CDC46
5	6	DNA replication licensing factor MCM6	polymer	821	93010.3	1	UniProt (Q14566) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) In PDB	Homo sapiens (human)	p105MCM
6	7	DNA replication licensing factor MCM7	polymer	719	81411.9	1	UniProt (P33993) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	CDC47 homolog,P1.1-MCM3
7	A	DNA polymerase alpha subunit B	polymer	598	66015.5	1	UniProt (Q14181) Pfam (PF08418) Pfam (PF22062) Pfam (PF04042) In PDB	Homo sapiens (human)	DNA polymerase alpha 70 kDa subunit
8	C	Cell division control protein 45 homolog	polymer	569	66016.9	1	UniProt (O75419) Pfam (PF02724) In PDB	Homo sapiens (human)	PORC-PI-1
9	D	DNA replication complex GINS protein PSF1	polymer	196	23022.5	1	UniProt (Q14691) Pfam (PF05916) In PDB	Homo sapiens (human)	GINS complex subunit 1
10	E	DNA replication complex GINS protein PSF2	polymer	185	21453.7	1	UniProt (Q9Y248) Pfam (PF05916) In PDB	Homo sapiens (human)	GINS complex subunit 2
11	F	DNA replication complex GINS protein PSF3	polymer	216	24562.6	1	UniProt (Q9BRX5) Pfam (PF22466) Pfam (PF05916) In PDB	Homo sapiens (human)	GINS complex subunit 3
12	G	DNA replication complex GINS protein SLD5	polymer	261	29983.0	1	UniProt (Q9BRT9) Pfam (PF05916) Pfam (PF16922) In PDB	Homo sapiens (human)	GINS complex subunit 4
13	K	Protein timeless homolog	polymer	1209	138903.0	1	UniProt (Q9UNS1) Pfam (PF04821) Pfam (PF05029) In PDB	Homo sapiens (human)	hTIM
14	L	TIMELESS-interacting protein	polymer	301	34600.2	1	UniProt (Q9BVW5) Pfam (PF07962) In PDB	Homo sapiens (human)
15	M	Leading strand DNA	polymer	84	26092.6	1		Homo sapiens
16	N	DNA Molecule	polymer	86	26402.9	1		Homo sapiens
17	P	DNA primase large subunit	polymer	509	58890.9	1	UniProt (P49643) Pfam (PF04104) In PDB	Homo sapiens (human)	DNA primase 58 kDa subunit,p58
18	Q	Claspin	polymer	1371	155184.7	1	UniProt (Q9HAW4) In PDB	Homo sapiens (human)	hClaspin
19	O	DNA primase small subunit	polymer	462	54242.6	1	UniProt (P49642) Pfam (PF01896) In PDB	Homo sapiens (human)	DNA primase 49 kDa subunit,p49
20	H, J, I	WD repeat and HMG-box DNA-binding protein 1	polymer	1160	129967.0	3	UniProt (O75717) Pfam (PF00400) Pfam (PF12894) Pfam (PF12341) Pfam (PF20946) In PDB	Homo sapiens (human)	Acidic nucleoplasmic DNA-binding protein 1,And-1
21	B	DNA polymerase alpha catalytic subunit	polymer	1504	170392.7	1	UniProt (P09884) Pfam (PF12254) Pfam (PF03104) Pfam (PF00136) Pfam (PF08996) In PDB	Homo sapiens (human)	DNA polymerase alpha catalytic subunit p180
22	5, 2, 3	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	3	Chemie (ANP)
23	3, 5, 2	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
24	4, 5, 6, 7	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

C: 1 - 134 (UniProt: O75419)
C: 135 - 566 (UniProt: O75419)

PDB	External Database	Details
Asp 135	-	linker
Tyr 135	-	linker
Lys 135	-	linker
Asp 135	-	linker
Asp 135	-	linker
Asp 135	-	linker

G: 1 - 223 (UniProt: Q9BRT9)

PDB	External Database	Details
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ala -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

K: 1 - 1208 (UniProt: Q9UNS1)

PDB	External Database	Details
Gly 0	-	expression tag

Q: 1 - 1339 (UniProt: Q9HAW4)

PDB	External Database	Details
Leu 1340	-	expression tag
Glu 1341	-	expression tag
Val 1342	-	expression tag
Leu 1343	-	expression tag
Phe 1344	-	expression tag
Gln 1345	-	expression tag
Gly 1346	-	expression tag
Pro 1347	-	expression tag
Asp 1348	-	expression tag
Tyr 1349	-	expression tag
Lys 1350	-	expression tag
Asp 1351	-	expression tag
Asp 1352	-	expression tag
Asp 1353	-	expression tag
Asp 1354	-	expression tag
Lys 1355	-	expression tag
Asp 1356	-	expression tag
Tyr 1357	-	expression tag
Lys 1358	-	expression tag
Asp 1359	-	expression tag
Asp 1360	-	expression tag
Asp 1361	-	expression tag
Asp 1362	-	expression tag
Lys 1363	-	expression tag
Asp 1364	-	expression tag
Tyr 1365	-	expression tag
Lys 1366	-	expression tag
Asp 1367	-	expression tag
Asp 1368	-	expression tag
Asp 1369	-	expression tag
Asp 1370	-	expression tag
Lys 1371	-	expression tag

O: 1 - 420 (UniProt: P49642)

PDB	External Database	Details
Met -41	-	initiating methionine
Ala -40	-	expression tag
Ser -39	-	expression tag
Ala -38	-	expression tag
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ala -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

H, J, I: 1 - 1129 (UniProt: O75717)

PDB	External Database	Details
Asp -30	-	expression tag
Tyr -29	-	expression tag
Lys -28	-	expression tag
Asp -27	-	expression tag
Asp -26	-	expression tag
Asp -25	-	expression tag
Asp -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
Tyr -21	-	expression tag
Lys -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

B: 1 - 1462 (UniProt: P09884)

PDB	External Database	Details
Met -41	-	initiating methionine
Ala -40	-	expression tag
Ser -39	-	expression tag
Ala -38	-	expression tag
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ala -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	1832323.4
Non-Polymers*	Number of molecules	10
	Total formula weight	1853.1
All*	Total formula weight	1834176.5

*Water molecules are not included.