8B7Q

Cryo-EM structure for the mouse LEPR-CRH2:Leptin:LEPR-Ig complex following symmetry expansion in combination with local refinement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leptin	polymer	174	18873.3	1	UniProt (P41160) Pfam (PF02024)	Mus musculus (house mouse)	Obesity factor
2	B, C (B, F)	Leptin receptor	polymer	868	97479.4	2	UniProt (P48356) Pfam (PF18589) Pfam (PF00041) Pfam (PF06328)	Mus musculus (house mouse)	LEP-R,B219,OB receptor,OB-R
3	D (F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A: 21 - 167 (UniProt: P41160)

PDB	External Database	Details
Met -6	-	initiating methionine
Gly -5	-	expression tag
Ser -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Pro 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Pro 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Glu 10	-	expression tag
Asn 11	-	expression tag
Leu 12	-	expression tag
Tyr 13	-	expression tag
Phe 14	-	expression tag
Gln 15	-	expression tag
Gly 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag
Thr 19	-	expression tag
Gly 20	-	expression tag
Gly 21	Ala 21	conflict

B, F: 22 - 839 (UniProt: P48356)

PDB	External Database	Details
Ser 840	-	expression tag
Thr 841	-	expression tag
Gly 842	-	expression tag
Gly 843	-	expression tag
Ser 844	-	expression tag
Gly 845	-	expression tag
Gly 846	-	expression tag
Ser 847	-	expression tag
Gly 848	-	expression tag
Gly 849	-	expression tag
Ser 850	-	expression tag
Gly 851	-	expression tag
Gly 852	-	expression tag
Ser 853	-	expression tag
Gly 854	-	expression tag
Gly 855	-	expression tag
Ser 856	-	expression tag
Arg 857	-	expression tag
Met 858	-	expression tag
Lys 859	-	expression tag
Gln 860	-	expression tag
Ile 861	-	expression tag
Glu 862	-	expression tag
Asp 863	-	expression tag
Lys 864	-	expression tag
Ile 865	-	expression tag
Glu 866	-	expression tag
Glu 867	-	expression tag
Ile 868	-	expression tag
Leu 869	-	expression tag
Ser 870	-	expression tag
Lys 871	-	expression tag
Ile 872	-	expression tag
Tyr 873	-	expression tag
His 874	-	expression tag
Ile 875	-	expression tag
Glu 876	-	expression tag
Asn 877	-	expression tag
Glu 878	-	expression tag
Ile 879	-	expression tag
Ala 880	-	expression tag
Arg 881	-	expression tag
Ile 882	-	expression tag
Lys 883	-	expression tag
Lys 884	-	expression tag
Leu 885	-	expression tag
Ile 886	-	expression tag
Gly 887	-	expression tag
Glu 888	-	expression tag
Arg 889	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	213832.1
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	214053.3

*Water molecules are not included.