8B7E

The crystal structure of N828V variant of DNA Pol Epsilon containing UTP in the polymerase active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA polymerase epsilon catalytic subunit A	polymer	1191	137058.6	2	UniProt (P21951) Pfam (PF03104) Pfam (PF22634)	Saccharomyces cerevisiae (baker's yeast)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
2	C, E (C, P)	Primer DNA sequence	polymer	11	3293.2	2		synthetic construct
3	D, F (D, T)	Template DNA sequence	polymer	16	4898.2	2		synthetic construct
4	G, J (A, B)	URIDINE 5'-TRIPHOSPHATE	non-polymer		484.1	2	Chemie (UTP)
5	H, I, K, L (A, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	M (T)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	N, O, P, Q, R (A, B, D, P, T)	water	water		18.0	82	Chemie (HOH)

Sequence modifications

A, B: 1 - 1186 (UniProt: P21951)

PDB	External Database	Details
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Ala 290	Asp 290	engineered mutation
Ala 292	Glu 292	engineered mutation
Val 828	Asn 828	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	290499.9
Non-Polymers*	Number of molecules	7
	Total formula weight	1220.7
All*	Total formula weight	291720.6

*Water molecules are not included.