8B7E

The crystal structure of N828V variant of DNA Pol Epsilon containing UTP in the polymerase active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 1 2 1
Unit cell lengths	154.360, 69.990, 158.320
Unit cell angles	90.00, 112.55, 90.00

Refinement procedure

Resolution	26.750 - 2.600
R-factor	0.1995
Rwork	0.198
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m8o
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.390	78.390	2.640
High resolution limit [Å]	2.600	14.240	2.600
Rmerge	0.166	0.044	1.245
Rmeas	0.189	0.053	1.406
Rpim	0.089	0.029	0.643
Total number of observations	405993	2238	20717
Number of reflections	95522	642	4681
<I/σ(I)>	6.8	13.3	1.4
Completeness [%]	98.9	98.2	99.9
Redundancy	4.3	3.5	4.4
CC(1/2)	0.994	0.986	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	10mM Tris, 15% PEG8K,10mM CaCl2