8B6K
The crystal structure of M644G variant of DNA Pol Epsilon containing dCTP in the polymerase active site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA polymerase epsilon catalytic subunit A | polymer | 1191 | 136999.4 | 2 | UniProt (P21951) Pfam (PF03104) Pfam (PF00136) In PDB | Saccharomyces cerevisiae (baker's yeast) | 3'-5' exodeoxyribonuclease,DNA polymerase II subunit A |
2 | P, C | Primer DNA sequence | polymer | 11 | 3293.2 | 2 | synthetic construct | ||
3 | T, D | Template DNA sequence | polymer | 16 | 4914.2 | 2 | synthetic construct | ||
4 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
5 | A, B | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 2 | Chemie (DCP) |
Sequence modifications
A, B: 1 - 1186 (UniProt: P21951)
PDB | External Database | Details |
---|---|---|
Gly -4 | - | expression tag |
Gly -3 | - | expression tag |
Asp -2 | - | expression tag |
Pro -1 | - | expression tag |
His 0 | - | expression tag |
Ala 290 | Asp 290 | engineered mutation |
Ala 292 | Glu 292 | engineered mutation |
Gly 644 | Met 644 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 290413.5 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 1014.5 | |
All* | Total formula weight | 291428.0 |