8B6K
The crystal structure of M644G variant of DNA Pol Epsilon containing dCTP in the polymerase active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9125 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 154.370, 70.420, 158.659 |
| Unit cell angles | 90.00, 112.69, 90.00 |
Refinement procedure
| Resolution | 86.720 - 2.500 |
| R-factor | 0.2297 |
| Rwork | 0.228 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m8o |
| Data reduction software | XDS (v2021) |
| Data scaling software | XSCALE (v2021) |
| Phasing software | PHASER (3.55) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.720 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.200 | 1.260 |
| Number of reflections | 109188 | 5329 |
| <I/σ(I)> | 5.4 | 1.1 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 3.9 | 3.6 |
| CC(1/2) | 0.984 | 0.080 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 10mM Tris, 15% PEG8K,10mM CaCl2 |
