8B6F
Cryo-EM structure of NADH:ubiquinone oxidoreductase (complex-I) from respiratory supercomplex of Tetrahymena thermophila
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A0) | Lipid-A-disaccharide synthase | polymer | 516 | 59307.3 | 1 | UniProt (Q22E24) Pfam (PF02684) | Tetrahymena thermophila SB210 | |
| 2 | B (A1) | NAD-dependent epimerase/dehydratase family protein | polymer | 362 | 41375.1 | 1 | UniProt (I7MLH2) Pfam (PF01370) | Tetrahymena thermophila SB210 | |
| 3 | C (A2) | DnaJ domain protein | polymer | 317 | 36878.4 | 1 | UniProt (Q22DR7) Pfam (PF00226) | Tetrahymena thermophila SB210 | |
| 4 | D (A3) | Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II | polymer | 333 | 36709.4 | 1 | UniProt (Q24HK5) Pfam (PF00501) | Tetrahymena thermophila SB210 | |
| 5 | E (A4) | RNase III domain-containing protein | polymer | 311 | 35409.2 | 1 | UniProt (I7LUQ4) | Tetrahymena thermophila SB210 | |
| 6 | F (A5) | 37S ribosomal protein S25, mitochondrial | polymer | 282 | 33201.8 | 1 | UniProt (I7MIJ7) | Tetrahymena thermophila SB210 | |
| 7 | G (A6) | Transmembrane protein, putative | polymer | 251 | 29389.7 | 1 | UniProt (Q24C39) | Tetrahymena thermophila SB210 | |
| 8 | H (A7) | CX9C domain-containing protein | polymer | 238 | 28412.8 | 1 | UniProt (I7MMF4) | Tetrahymena thermophila SB210 | |
| 9 | I (A8) | NDUTT15 | polymer | 217 | 25493.3 | 1 | Tetrahymena thermophila SB210 | ||
| 10 | J (A9) | Transmembrane protein, putative | polymer | 231 | 26545.1 | 1 | UniProt (Q24F24) | Tetrahymena thermophila SB210 | |
| 11 | K (AA) | NADH dehydrogenase subunit 5 | polymer | 750 | 88179.8 | 1 | UniProt (Q950Z0) Pfam (PF00361) | Tetrahymena thermophila SB210 | |
| 12 | L (AB) | NADH-ubiquinone oxidoreductase 75 kDa subunit | polymer | 718 | 80580.8 | 1 | UniProt (Q23KA9) Pfam (PF00384) Pfam (PF09326) Pfam (PF13510) Pfam (PF10588) Pfam (PF22117) Pfam (PF22151) | Tetrahymena thermophila SB210 | |
| 13 | M (AC) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 505 | 58690.0 | 1 | UniProt (Q950X9) Pfam (PF00361) | Tetrahymena thermophila SB210 | |
| 14 | N (AD) | NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | polymer | 474 | 52313.7 | 1 | UniProt (Q23KE4) Pfam (PF01512) Pfam (PF22461) Pfam (PF10531) Pfam (PF10589) | Tetrahymena thermophila SB210 | |
| 15 | O (AE) | NADH dehydrogenase subunit 7 | polymer | 442 | 51227.5 | 1 | UniProt (Q951B1) Pfam (PF00346) | Tetrahymena thermophila SB210 | |
| 16 | P (AF) | Ymf65 | polymer | 360 | 43146.2 | 1 | UniProt (Q951A3) Pfam (PF00361) | Tetrahymena thermophila SB210 | |
| 17 | Q (AG) | Transcription factor apfi protein, putative | polymer | 346 | 39793.4 | 1 | UniProt (I7M8Q7) Pfam (PF00132) | Tetrahymena thermophila SB210 | |
| 18 | R (AH) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 284 | 32636.8 | 1 | UniProt (Q950Y3) Pfam (PF00146) | Tetrahymena thermophila SB210 | |
| 19 | S (AI) | NADH-ubiquinone oxidoreductase 24 kDa subunit | polymer | 274 | 31216.7 | 1 | UniProt (I7MEP0) Pfam (PF01257) | Tetrahymena thermophila SB210 | |
| 20 | T (AJ) | Ymf62 | polymer | 255 | 30448.5 | 1 | UniProt (Q950Y2) | Tetrahymena thermophila SB210 | |
| 21 | U (AK) | Gamma-carbonic anhydrase | polymer | 257 | 28216.7 | 1 | UniProt (Q22XU5) Pfam (PF00132) | Tetrahymena thermophila SB210 | |
| 22 | V (AL) | NADH-ubiquinone oxidoreductase 1, chain, putative | polymer | 236 | 28053.5 | 1 | UniProt (I7MDW5) Pfam (PF12838) | Tetrahymena thermophila SB210 | |
| 23 | W (AM) | Gamma-carbonic anhydrase | polymer | 233 | 25396.0 | 1 | UniProt (I7M6S0) Pfam (PF21711) | Tetrahymena thermophila SB210 | |
| 24 | X (AN) | ETC complex I subunit motif protein | polymer | 206 | 24277.9 | 1 | UniProt (Q23ND5) Pfam (PF04716) | Tetrahymena thermophila SB210 | |
| 25 | Y (AO) | NADH dehydrogenase subunit 9 | polymer | 198 | 23830.3 | 1 | UniProt (Q950Z3) Pfam (PF00329) | Tetrahymena thermophila SB210 | |
| 26 | Z (AP) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | polymer | 194 | 22890.6 | 1 | UniProt (A4VDQ6) Pfam (PF05071) | Tetrahymena thermophila SB210 | |
| 27 | AA (AQ) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial | polymer | 189 | 22698.3 | 1 | UniProt (Q233X7) | Tetrahymena thermophila SB210 | |
| 28 | BA (AR) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial | polymer | 185 | 21642.4 | 1 | UniProt (I7MK61) Pfam (PF04800) | Tetrahymena thermophila SB210 | |
| 29 | CA (AS) | NADH dehydrogenase, putative | polymer | 172 | 20167.9 | 1 | UniProt (I7M2Y3) | Tetrahymena thermophila SB210 | |
| 30 | DA (AT) | NADH dehydrogenase subunit 10 | polymer | 162 | 18324.3 | 1 | UniProt (Q951B4) Pfam (PF01058) | Tetrahymena thermophila SB210 | |
| 31 | EA (AU) | NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit | polymer | 150 | 17381.8 | 1 | UniProt (I7LT42) Pfam (PF10785) | Tetrahymena thermophila SB210 | |
| 32 | FA (AV) | Acyl carrier protein | polymer | 138 | 16249.4 | 1 | UniProt (Q22XT6) Pfam (PF00550) | Tetrahymena thermophila SB210 | |
| 33 | GA (AW) | Acyl carrier protein | polymer | 133 | 15428.4 | 1 | UniProt (I7MD12) Pfam (PF00550) | Tetrahymena thermophila SB210 | |
| 34 | HA (AX) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 121 | 14416.9 | 1 | UniProt (Q950Z7) Pfam (PF00507) | Tetrahymena thermophila SB210 | |
| 35 | IA (AY) | Ymf58 | polymer | 116 | 13509.9 | 1 | UniProt (Q950Z5) | Tetrahymena thermophila SB210 | |
| 36 | JA (AZ) | Ribosomal protein L51/S25/CI-B8 domain protein | polymer | 103 | 12157.8 | 1 | UniProt (I7MA77) Pfam (PF05047) | Tetrahymena thermophila SB210 | |
| 37 | KA (B0) | Transmembrane protein, putative | polymer | 94 | 11451.2 | 1 | UniProt (I7MI60) | Tetrahymena thermophila SB210 | |
| 38 | LA (B1) | ATP synthase subunit e, mitochondrial | polymer | 93 | 11283.5 | 1 | UniProt (Q22E95) | Tetrahymena thermophila SB210 | |
| 39 | MA (B2) | GRAM domain protein | polymer | 94 | 10920.4 | 1 | UniProt (W7X4R4) | Tetrahymena thermophila SB210 | |
| 40 | NA (B3) | Transmembrane protein, putative | polymer | 83 | 9942.4 | 1 | UniProt (A4VD20) | Tetrahymena thermophila SB210 | |
| 41 | OA (B4) | Transmembrane protein, putative | polymer | 73 | 8787.1 | 1 | UniProt (Q22DC2) | Tetrahymena thermophila SB210 | |
| 42 | PA (B5) | Transmembrane protein, putative | polymer | 71 | 8445.9 | 1 | UniProt (I7MIK1) | Tetrahymena thermophila SB210 | |
| 43 | QA (B6) | ND1b | polymer | 59 | 7238.8 | 1 | Tetrahymena thermophila SB210 | ||
| 44 | RA (BA) | Transmembrane protein | polymer | 212 | 24823.3 | 1 | UniProt (I7MIE0) | Tetrahymena thermophila SB210 | |
| 45 | SA (BB) | Transmembrane protein, putative | polymer | 214 | 24419.2 | 1 | UniProt (Q22Z32) | Tetrahymena thermophila SB210 | |
| 46 | TA (BC) | NDUB8 | polymer | 207 | 24150.5 | 1 | UniProt (I7M855) | Tetrahymena thermophila SB210 | |
| 47 | UA (BD) | Transmembrane protein, putative | polymer | 205 | 23971.0 | 1 | UniProt (I7LT77) | Tetrahymena thermophila SB210 | |
| 48 | VA (BE) | NDUPH2 | polymer | 189 | 22446.6 | 1 | UniProt (Q23KE0) | Tetrahymena thermophila SB210 | |
| 49 | WA (BF) | NDUB10 | polymer | 188 | 22167.1 | 1 | UniProt (Q23KG0) | Tetrahymena thermophila SB210 | |
| 50 | XA (BG) | NDUA13 | polymer | 175 | 21045.6 | 1 | UniProt (I7M2U4) | Tetrahymena thermophila SB210 | |
| 51 | YA (BH) | NADH dehydrogenase subunit 2 | polymer | 178 | 20899.9 | 1 | UniProt (Q951B2) Pfam (PF00361) | Tetrahymena thermophila SB210 | |
| 52 | ZA (BI) | 2 iron, 2 sulfur cluster-binding protein | polymer | 172 | 19564.2 | 1 | UniProt (Q22W11) Pfam (PF00111) | Tetrahymena thermophila SB210 | |
| 53 | AB (BJ) | Thioredoxin | polymer | 166 | 19540.2 | 1 | UniProt (Q22AI5) Pfam (PF00085) | Tetrahymena thermophila SB210 | |
| 54 | BB (BK) | COX assembly mitochondrial protein | polymer | 144 | 17078.3 | 1 | UniProt (I7M1N8) | Tetrahymena thermophila SB210 | |
| 55 | CB (BL) | Transmembrane protein, putative | polymer | 143 | 16909.2 | 1 | UniProt (Q22HE4) | Tetrahymena thermophila SB210 | |
| 56 | DB (BM) | Transmembrane protein, putative | polymer | 135 | 15831.7 | 1 | UniProt (I7M9B3) | Tetrahymena thermophila SB210 | |
| 57 | EB (BN) | PH domain-containing protein | polymer | 135 | 15785.2 | 1 | UniProt (Q22SC4) | Tetrahymena thermophila SB210 | |
| 58 | FB (BO) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 | polymer | 136 | 15689.8 | 1 | UniProt (Q23B10) | Tetrahymena thermophila SB210 | |
| 59 | GB (BP) | NDUB6 | polymer | 129 | 15444.3 | 1 | UniProt (Q231G0) | Tetrahymena thermophila SB210 | |
| 60 | HB (BQ) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 | polymer | 127 | 15193.6 | 1 | UniProt (I7MAF0) | Tetrahymena thermophila SB210 | |
| 61 | IB (BR) | Zinc-finger protein | polymer | 132 | 15141.3 | 1 | UniProt (I7MK02) Pfam (PF10276) | Tetrahymena thermophila SB210 | |
| 62 | JB (BS) | NDUB4 | polymer | 126 | 14410.4 | 1 | UniProt (I7MG29) | Tetrahymena thermophila SB210 | |
| 63 | KB (BT) | NDUB15 | polymer | 125 | 14963.5 | 1 | Tetrahymena thermophila SB210 | ||
| 64 | LB (BU) | NDUTT16 | polymer | 134 | 15334.4 | 1 | Tetrahymena thermophila SB210 | ||
| 65 | MB (BV) | NDUTT17 | polymer | 125 | 13983.0 | 1 | Tetrahymena thermophila SB210 | ||
| 66 | NB (BW) | CHCH domain-containing protein | polymer | 120 | 13747.4 | 1 | UniProt (I7MIM0) | Tetrahymena thermophila SB210 | |
| 67 | OB (BX) | Transmembrane protein, putative | polymer | 113 | 13341.0 | 1 | UniProt (Q22T55) | Tetrahymena thermophila SB210 | |
| 68 | PB (BY) | Ymf57 | polymer | 100 | 12473.9 | 1 | UniProt (Q951C2) | Tetrahymena thermophila SB210 | |
| 69 | QB (BZ) | Complex I-MNLL | polymer | 102 | 12103.0 | 1 | UniProt (Q22W63) | Tetrahymena thermophila SB210 | |
| 70 | AD, AE, BD, BE, CE... (AF, BC, BE, BG, BL...) | CARDIOLIPIN | non-polymer | 1464.0 | 22 | Chemie (CDL) | |||
| 71 | TB (A0) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 1 | Chemie (UDP) | |||
| 72 | GC, UB (A8, A0) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 73 | BC, CD, DC, EC, ED... (A2, AH, A6, AJ, A9...) | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 27 | Chemie (PC1) | |||
| 74 | WB (A1) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 75 | AC, FD, GE, IC, JC... (A2, AJ, BP, A9, BA) | 1,2-Distearoyl-sn-glycerophosphoethanolamine | non-polymer | 748.1 | 6 | Chemie (3PE) | |||
| 76 | CC, FE, JD, MC (A6, BN, AL, AA) | 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE | non-polymer | 648.9 | 4 | Chemie (LPP) | |||
| 77 | FC, ND (A8, AQ) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 78 | DD, DE, TC (AI, BI, AB) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 3 | Chemie (FES) | |||
| 79 | GD, HD, QD, UC, VC... (AL, AT, AB, AD) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 80 | ZC (AD) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) | |||
| 81 | PD (AS) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 1 | Chemie (8Q1) | |||
| 82 | IE (BR) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
AF: 1 - 360 (UniProt: Q951A3)
| PDB | External Database | Details |
|---|---|---|
| Val 208 | Gly 208 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 69 |
| Total formula weight | 1714124.8 | |
| Non-Polymers* | Number of molecules | 77 |
| Total formula weight | 66379.1 | |
| All* | Total formula weight | 1780503.9 |
