8B5M

Crystal structure of GH47 alpha-1,2-mannosidase from Caulobacter K31 strain in complex with cyclosulfamidate inhibitor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mannosyl-oligosaccharide 1,2-alpha-mannosidase	polymer	447	50520.6	1	UniProt (B0SWV2) Pfam (PF01532) In PDB	Caulobacter
2	A	(3aR,4S,5S,6R,7R,7aS)-7-(hydroxymethyl)-2,2-bis(oxidanylidene)-3a,4,5,6,7,7a-hexahydro-3H-benzo[d][1,2,3]oxathiazole-4,5,6-triol	non-polymer		255.2	1	Chemie (5OV)
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5		water	water		18.0	687	Chemie (HOH)

Sequence modifications

A: 27 - 462 (UniProt: B0SWV2)

PDB	External Database	Details
Met 24	-	initiating methionine
Ala 25	-	expression tag
Ser 26	-	expression tag
Ser 310	Leu 310	conflict
Leu 463	-	expression tag
Glu 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50520.6
Non-Polymers*	Number of molecules	4
	Total formula weight	358.4
All*	Total formula weight	50879.0

*Water molecules are not included.