8B37

Crystal structure of Pyrobaculum aerophilum potassium-independent proton pumping membrane integral pyrophosphatase in complex with imidodiphosphate and magnesium, and with bound sulphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	K(+)-insensitive pyrophosphate-energized proton pump	polymer	729	76386.6	2	UniProt (Q8ZWI8) Pfam (PF03030)	Pyrobaculum aerophilum	Membrane-bound proton-translocating pyrophosphatase,Pyrophosphate-energized inorganic pyrophosphatase,H(+)-PPase
2	C, D, E, F, G... (A, B)	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
3	H, N (A, B)	IMIDODIPHOSPHORIC ACID	non-polymer		177.0	2	Chemie (2PN)
4	O (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	P, Q (A, B)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A, B: 2 - 721 (UniProt: Q8ZWI8)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	152773.3
Non-Polymers*	Number of molecules	13
	Total formula weight	693.1
All*	Total formula weight	153466.4

*Water molecules are not included.