8B01

Cryo-EM structure of the Tripartite ATP-independent Periplasmic (TRAP) transporter SiaQM from Photobacterium profundum in a nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative TRAP-type C4-dicarboxylate transport system, small permease component	polymer	170	19748.4	1	UniProt (Q6LPW0) Pfam (PF04290)	Photobacterium profundum SS9
2	B (B)	Putative TRAP-type C4-dicarboxylate transport system, large permease component	polymer	427	45573.6	1	UniProt (Q6LPW1) Pfam (PF06808)	Photobacterium profundum SS9
3	C (C)	Megabody c7HopQ	polymer	510	54867.1	1	UniProt (B5Z8H1) Pfam (PF18304)	Helicobacter pylori
4	D, J, K, L, M... (A, B)	N-OCTANE	non-polymer		114.2	6	Chemie (OCT)
5	E, Q (A, B)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	2	Chemie (PTY)
6	F (A)	DOCOSANE	non-polymer		310.6	1	Chemie (TWT)
7	G, P (A, B)	DECANE	non-polymer		142.3	2	Chemie (D10)
8	H, I (B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
9	O (B)	TRIDECANE	non-polymer		184.4	1	Chemie (TRD)
10	R (B)	HEXANE	non-polymer		86.2	1	Chemie (HEX)

Sequence modifications

A: 1 - 169 (UniProt: Q6LPW0)

PDB	External Database	Details
Glu 170	-	expression tag

B: 1 - 426 (UniProt: Q6LPW1)

PDB	External Database	Details
Leu 427	-	expression tag

C: -377 - -157 (UniProt: B5Z8H1)

PDB	External Database	Details
Gln -389	-	expression tag
Val -388	-	expression tag
Gln -387	-	expression tag
Leu -386	-	expression tag
Gln -385	-	expression tag
Glu -384	-	expression tag
Ser -383	-	expression tag
Gly -382	-	expression tag
Gly -381	-	expression tag
Gly -380	-	expression tag
Leu -379	-	expression tag
Val -378	-	expression tag

C: -156 - 120 (PDB: 8B01)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	120189.1
Non-Polymers*	Number of molecules	15
	Total formula weight	3065.1
All*	Total formula weight	123254.2

*Water molecules are not included.