8AW3

Cryo-EM structure of the Tb ADAT2/3 deaminase in complex with tRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (1)	RNA (75-MER)	polymer	75	24132.3	1	GenBank (62359426)	Trypanosoma brucei
2	B (2)	Deaminase, putative	polymer	221	23547.5	1	UniProt (Q57W17) Pfam (PF00383)	Trypanosoma brucei brucei
3	C (3)	Deaminase, putative	polymer	369	40600.3	1	UniProt (Q381Q7) Pfam (PF14437)	Trypanosoma brucei brucei
4	D, E (2, 3)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

1: 1 - 74 (GenBank: 62359426)
1: 75 - 76 (PDB: 8AW3)
2: 1 - 221 (UniProt: Q57W17)

PDB	External Database	Details
Gly 87	Ala 87	engineered mutation
Ser 117	Cys 117	engineered mutation

3: 1 - 365 (UniProt: Q381Q7)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag
Lys 102	Asn 102	variant
Val 105	Ile 105	variant
Val 167	Ala 167	variant
Val 176	Met 176	variant

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	88280.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	88410.9

*Water molecules are not included.