8ASN

Crystal structure of the apo human TTL in complex with tubulin-stathmin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (cattle)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (cattle)
3	E (E)	Stathmin-4	polymer	143	16883.2	1	UniProt (P63043) Pfam (PF00836)	Rattus norvegicus (Norway rat)	Stathmin-like protein B3,RB3
4	F, G, H, I (F, G, H, I)	Tubulin--tyrosine ligase	polymer	383	43984.9	4	UniProt (Q8NG68) Pfam (PF03133)	Homo sapiens (human)	TTL
5	J, M, N, O (A, B, C)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	K, P (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
7	L, Q (B, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
8	R, S, T, U (F, G, H, I)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	4	Chemie (ACP)
9	AA, BA, CA, DA, V... (F, G, H, I, A...)	water	water		18.0	458	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	-	expression tag
Trp 20	Phe 64	conflict

F, G, H, I: 3 - 379 (UniProt: Q8NG68)

PDB	External Database	Details
Gly 2	-	expression tag
Glu 380	-	expression tag
Asn 381	-	expression tag
Leu 382	-	expression tag
Tyr 383	-	expression tag
Phe 384	-	expression tag
Gln 385	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	393231.3
Non-Polymers*	Number of molecules	12
	Total formula weight	4050.8
All*	Total formula weight	397282.1

*Water molecules are not included.