8ASN

Crystal structure of the apo human TTL in complex with tubulin-stathmin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98008
Spacegroup name	P 1 21 1
Unit cell lengths	82.553, 199.636, 134.669
Unit cell angles	90.00, 97.02, 90.00

Refinement procedure

Resolution	133.660 - 2.571
R-factor	0.2443
Rwork	0.243
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sa0
RMSD bond length	0.006
RMSD bond angle	0.820
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	133.659	2.803
High resolution limit [Å]	2.571	2.571
Number of reflections	89154	4458
<I/σ(I)>	11.2
Completeness [%]	92.6
Redundancy	7
CC(1/2)	0.998	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	4 % Tacsimate pH 6.0, 12 % PEG3350