8AS9

Crystal structure of the talin-KANK1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	B-cell lymphoma 6 protein	polymer	137	15603.0	2	UniProt (P41182) Pfam (PF00651)	Homo sapiens (human)	BCL-6,B-cell lymphoma 5 protein,BCL-5,Protein LAZ-3,Zinc finger and BTB domain-containing protein 27,Zinc finger protein 51
2	C (D)	KN-motif NCoR1 BBD fusion,Nuclear receptor corepressor 1	polymer	51	5781.6	1	UniProt (Q8BRZ8) UniProt (O75376) Pfam (PF12075) UniProt (by SIFTS) (Q14678)	Homo sapiens	N-CoR,N-CoR1
3	D (C)	Talin-1	polymer	309	32574.7	1	UniProt (P26039)	Mus musculus (mouse)
4	E, F, H, I (A, C)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	G (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)

Sequence modifications

A, B: 6 - 129 (UniProt: P41182)

PDB	External Database	Details
Gly -7	-	expression tag
Pro -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Asp -3	-	expression tag
Leu -2	-	expression tag
Tyr -1	-	expression tag
Leu 0	-	expression tag
Arg 1	-	expression tag
Pro 2	-	expression tag
Gly 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Gln 8	Cys 8	engineered mutation
Arg 67	Cys 67	engineered mutation
Asn 84	Cys 84	engineered mutation

D: 1 - 31 (UniProt: Q8BRZ8)

PDB	External Database	Details
Gly 32	-	linker
Gly 33	-	linker
Gly 34	-	linker
Gly 35	-	linker

D: 36 - 51 (UniProt: O75376)
C: 1359 - 1659 (UniProt: P26039)

PDB	External Database	Details
Gly 1351	-	expression tag
Ile 1352	-	expression tag
Asp 1353	-	expression tag
Pro 1354	-	expression tag
Phe 1355	-	expression tag
Thr 1356	-	expression tag
Lys 1357	-	expression tag
His 1358	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69562.3
Non-Polymers*	Number of molecules	5
	Total formula weight	476.3
All*	Total formula weight	70038.6

*Water molecules are not included.