8AOW

CryoEM structure of the Chikungunya virus nsP1 capping pores in complex with m7GTP and SAH ligands

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, C, E, G, I...)	mRNA-capping enzyme nsP1	polymer	543	60946.5	12	UniProt (Q8JUX6) Pfam (PF01660)	Chikungunya virus strain S27-African prototype	Non-structural protein 1
2	BB, CA, GB, HA, LB... (Q, G, S, I, V...)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
3	CB, DA, HB, IA, MB... (Q, G, S, I, V...)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	12	Chemie (SAH)
4	DB, EA, IB, JA, NB... (Q, G, S, I, V...)	7-METHYL-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		538.2	12	Chemie (MGP)
5	AA, AB, BA, EB, FA... (E, Q, G, S, I...)	MAGNESIUM ION	non-polymer		24.3	24	Chemie (MG)
6	AC, BC, CC, DC, EC... (M, O, Q, S, V...)	water	water		18.0	324	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O, Q, S, V, X: 1 - 535 (UniProt: Q8JUX6)

PDB	External Database	Details
Ala 536	-	expression tag
Ala 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	731357.7
Non-Polymers*	Number of molecules	60
	Total formula weight	12439.7
All*	Total formula weight	743797.4

*Water molecules are not included.