8AMS

Complex of human TRIM2 RING domain, UBCH5C, and Ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ubiquitin-conjugating enzyme E2 D3	polymer	149	16955.4	2	UniProt (P61077) Pfam (PF00179) In PDB	Homo sapiens (human)	(E3-independent) E2 ubiquitin-conjugating enzyme D3,E2 ubiquitin-conjugating enzyme D3,Ubiquitin carrier protein D3,Ubiquitin-conjugating enzyme E2(17)KB 3,Ubiquitin-conjugating enzyme E2-17 kDa 3,Ubiquitin-protein ligase D3
2	C, D	Tripartite motif-containing protein 2	polymer	153	16914.5	2	UniProt (Q9C040) Pfam (PF13445) Pfam (PF00643) In PDB	Homo sapiens (human)	E3 ubiquitin-protein ligase TRIM2,RING finger protein 86,RING-type E3 ubiquitin transferase TRIM2
3	E	Polyubiquitin-C	polymer	101	11639.2	1	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)
4	A, B, C, D	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
5	C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	D	3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL	non-polymer		370.4	1	Chemie (PE8)
7		water	water		18.0	158	Chemie (HOH)

Sequence modifications

A, B: 1 - 147 (UniProt: P61077)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Arg 22	Ser 22	engineered mutation
Ser 85	Cys 85	engineered mutation

C, D: 8 - 157 (UniProt: Q9C040)

PDB	External Database	Details
Gly 5	-	expression tag
Ala 6	-	expression tag
Met 7	-	expression tag

E: 0 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Met -24	-	initiating methionine
Lys -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Met -15	-	expression tag
Ser -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	79379.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1460.9
All*	Total formula weight	80839.9

*Water molecules are not included.