8AMS

Complex of human TRIM2 RING domain, UBCH5C, and Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967700
Spacegroup name	P 21 21 21
Unit cell lengths	68.120, 69.270, 151.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.920 - 2.400
R-factor	0.215
Rwork	0.214
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RING domain of TRIM2 Ubiquitin
RMSD bond length	0.012
RMSD bond angle	1.678
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	XDS (Jan 10, 2022)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.920	2.377
High resolution limit [Å]	2.295	2.295
Number of reflections	32226	2629
<I/σ(I)>	14.54
Completeness [%]	97.5
Redundancy	12.7
CC(1/2)	0.993	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.2 M sodium formate, 0.1 M bis-tris propane, 20% PEG 3350