8ALK
Structure of the Legionella phosphocholine hydrolase Lem3 in complex with its substrate Rab1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phosphocholine hydrolase Lem3 | polymer | 469 | 52900.6 | 1 | UniProt (Q5ZXN5) | Legionella pneumophila | Dephosphocholinase Lem3 |
| 2 | B (B) | Ras-related protein Rab-1B | polymer | 175 | 19725.4 | 1 | UniProt (Q9H0U4) Pfam (PF00071) | Homo sapiens (human) | |
| 3 | C, D, E, G (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 4 | F (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 5 | H (B) | 2-[[[5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxyethyl-[3-(2-chloranylethanoylamino)propyl]-dimethyl-azanium | non-polymer | 608.9 | 1 | Chemie (OJU) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 76 | Chemie (HOH) |
Sequence modifications
A: 21 - 486 (UniProt: Q5ZXN5)
B: 3 - 174 (UniProt: Q9H0U4)
| PDB | External Database | Details |
|---|---|---|
| Gly 18 | - | expression tag |
| His 19 | - | expression tag |
| Met 20 | - | expression tag |
| Ser 134 | Cys 134 | engineered mutation |
| Ser 209 | Cys 209 | engineered mutation |
| Cys 391 | Thr 391 | engineered mutation |
| Ser 456 | Cys 456 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Met 2 | - | expression tag |
| Thr 76 | Ser 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 72626.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1212.4 | |
| All* | Total formula weight | 73838.4 |
