8AKJ
Acyl-enzyme complex of cephalothin bound to deacylation mutant KPC-2 (E166Q)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Carbapenem-hydrolyzing beta-lactamase KPC | polymer | 290 | 30805.6 | 1 | UniProt (Q9F663) Pfam (PF00144) Pfam (PF13354) In PDB | Klebsiella pneumoniae | Carbapenem-hydrolyzing beta-lactamase KPC-1 |
| 2 | A | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 4 | A | 5-METHYLENE-2-[2-OXO-1-(2-THIOPHEN-2-YL-ACETYLAMINO)-ETHYL]-5,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC ACID | non-polymer | 338.4 | 1 | Chemie (CEO) | |||
| 5 | water | water | 18.0 | 248 | Chemie (HOH) |
Sequence modifications
A: 25 - 295 (UniProt: Q9F663)
| PDB | External Database | Details |
|---|---|---|
| Met 4 | - | initiating methionine |
| Gly 5 | - | expression tag |
| Ser 6 | - | expression tag |
| Ser 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| Ser 14 | - | expression tag |
| Ser 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Leu 17 | - | expression tag |
| Val 18 | - | expression tag |
| Pro 19 | - | expression tag |
| Arg 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Ser 22 | - | expression tag |
| His 23 | - | expression tag |
| Met 24 | - | expression tag |
| Gln 166 | Glu 165 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 30805.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 902.9 | |
| All* | Total formula weight | 31708.5 |
