8AG0

Crystal structure of mutant PRELID3a-TRIAP1 complex - R53E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	PRELI domain containing protein 3A	polymer	186	20961.9	1	UniProt (Q96N28) Pfam (PF04707)	Homo sapiens (human)	Protein slowmo homolog 1
2	B (B)	Maltose/maltodextrin-binding periplasmic protein,TP53-regulated inhibitor of apoptosis 1	polymer	446	48939.3	1	UniProt (P0AEX9) UniProt (O43715) Pfam (PF01547) Pfam (PF05254)	Homo sapiens (human)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein 15E1.1,WF-1,p53-inducible cell-survival factor,p53CSV
3	C (D)	alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G66120GK)
4	D, E (A, B)	water	water		18.0	49	Chemie (HOH)

Sequence modifications

A: 1 - 172 (UniProt: Q96N28)

PDB	External Database	Details
Met -13	-	initiating methionine
Ala -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Glu 53	Arg 53	engineered mutation

B: 1 - 361 (UniProt: P0AEX9)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 82	Asp 108	conflict
Ala 83	Lys 109	conflict
Ala 172	Glu 198	conflict
Ala 173	Asn 199	conflict
Ala 239	Lys 265	conflict
Ala 359	Glu 385	conflict
Ala 362	-	linker
Ala 363	-	linker
Ala 364	-	linker
Gln 365	-	linker
Thr 366	-	linker
Asn 367	-	linker
Ala 368	-	linker
Ala 369	-	linker
Ala 370	-	linker

B: 371 - 445 (UniProt: O43715)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69901.2
Branched	Number of molecules	1
	Total formula weight	342.3
All*	Total formula weight	70243.5

*Water molecules are not included.