8AG0
Crystal structure of mutant PRELID3a-TRIAP1 complex - R53E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.300, 66.815, 82.140 |
| Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
| Resolution | 45.410 - 2.700 |
| R-factor | 0.2184 |
| Rwork | 0.213 |
| R-free | 0.30360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zxv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.066 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.099 | 0.620 |
| Number of reflections | 18444 | 2445 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | Sodium acetate pH 4.6, 8% (w/v) PEG 4000 |
