8AAJ

Crystal structure of the Pyrococcus abyssi RPA (apo form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Replication factor A	polymer	358	41009.0	1	UniProt (G8ZHS0) Pfam (PF01336) Pfam (PF08646) UniProt (by SIFTS) (Q9V1Y9) In PDB	Pyrococcus abyssi GE5
2	B	RPA32 subunit of the hetero-oligomeric complex involved in homologous recombination	polymer	268	31376.2	1	UniProt (Q9V1Z1) Pfam (PF01336) In PDB	Pyrococcus abyssi GE5
3	C	RPA14 subunit of the hetero-oligomeric complex involved in homologous recombination	polymer	122	14008.9	1	UniProt (Q9V1Z0) In PDB	Pyrococcus abyssi GE5
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A, B, C	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)

Sequence modifications

A: 1 - 358 (UniProt: G8ZHS0)

PDB	External Database	Details
Ser 2	Thr 2	engineered mutation

B: 1 - 267 (UniProt: Q9V1Z1)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	Met 5	engineered mutation

C: 2 - 117 (UniProt: Q9V1Z0)

PDB	External Database	Details
Gly -4	-	expression tag
Thr -3	-	expression tag
Gly -2	-	expression tag
Asp -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	86394.0
Non-Polymers*	Number of molecules	12
	Total formula weight	1122.1
All*	Total formula weight	87516.1

*Water molecules are not included.